6 Agilent Cary 8454 UV-Visible Spectroscopy System Operator’s Manual
Contents
Standard Mode Data Processing 40
3 Installation and Start Up 47
Installation Summary for Your General Purpose UV-Visible System 48
General 48
Spectrophotometer 48
PC 49
Starting a Measurement Session 50
4 Good Measurement Practices 51
General Considerations 52
Spectrophotometer Design 52
Making Measurements 52
Sample Cell Material 53
Optical Specifications of Cells 54
Apertured Cells 55
Flow Cells 56
Handling and Maintaining Cells 57
Solvents 59
Sample Preparation 60
Photosensitive Samples 61
Stirring and Temperature Control 62
Checklist for Best Results 62
Inserting a Cell 65
5 Using your UV-Visible Spectroscopy System 67
Starting Your First Measurement Session 68
Starting Your UV-Visible Software 70
Measuring Caffeine Absorbance at 273 nm 71
Saving Your Parameters as a Method 74
Retrieving and Printing a Method 76