Agilent 1200 Series Valves 41
Valve Applications 4
Alternating Column Regeneration
If the valve is switched to position 1 the eluent pump delivers the mobile
phase through the injection loop of the autosampler into port 2 of the Agilent
1200 Series Valve. The sample is separated on column 1 and analyzed by the
detector. Simultaneously a second regeneration pump flushes and equilibrates
column 2.
After the analysis of the sample is finished on column 1, column 2 is prepared
for an immediate injection. The 2 position/ 10 port valve is switched and the
sample can be injected and analyzed. While the analysis is running on column
2, column 1 is now regenerated and will be ready to use immediately after the
sample run on column 2 is finished.
The advantage of this technique is visualized in Figure 19, where the time of
analysis with sequential column regeneration is compared to time of analysis
using alternating column.
A detailed description how to setup alternating column regeneration with an
Agilent 1200 Series LC system is provided in the application notes with the
publication numbers 5988-7831EN and 5988-7895EN.
We recommend to use the Agilent Technologies Capillary kits for Alternating
Column Regeneration. These kits contain all capillaries, fittings and ferrules
that are required for the setup displayed in Figure 18 on page 40. Agilent
Technologies offers one kit for analytical scale applications (flow rates < 10
ml/min), that contain the capillaries listed in Table 7 on page 42 and fittings
listed in Table 8 on page 42. Another kit is suited for preparative scale
applications (flow rates < 100 ml/min). Its capillaries are listed in Table 9 on
page 43 and its fitting are listed in Table 10 on page 43.
Figure 19 Alternating column regeneration (time scheme)
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