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Amersham Frac-950 User Manual

Amersham Frac-950
60 pages
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Fraction Collector
Frac-950
18-1139-56
User Manual
18113956_Front.fm Page 1 Wednesday, May 22, 2002 2:12 PM

Table of Contents

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Amersham Frac-950 Specifications

General IconGeneral
TypeFraction Collector
ModelFrac-950
InterfaceRS-232
Collection MethodTime-based
Collection Volume Range0.1 mL to 999.9 ml
Tube Size Compatibility10 mm to 25 mm diameter
Collection Modespeak
Power Requirements100-240 V, 50/60 Hz
ManufacturerAmersham Biosciences

Summary

Introduction

1.1 General

Overview of the Frac-950's functionality and intended use.

1.2 Safety

Critical safety precautions and warnings for operating the Frac-950.

Installation

2.1 Unpacking

Instructions for unpacking and inspecting the Frac-950 unit.

2.2 General Precautions

Guidelines on environmental conditions and placement for the unit.

2.3 Assembling Tube Racks

Introduces the different types of tube racks and their configurations.

2.4 Installing a Tube Rack

Step-by-step guide for fitting standard and prep mode tube racks into the holder.

2.5 Installing Capillaries

Instructions for connecting capillaries for DropSync and accumulator use.

2.6 Waste Handling

Procedures for connecting and managing waste tubing.

2.7 Connecting Electrical Signal Cables

How to connect the DropSync and Accumulator cables.

2.8 Connecting to a UniNet 1 Communication Link

Details on establishing communication with the control system.

2.9 Connecting to a Supply Voltage

Instructions for connecting the unit to the mains power supply.

2.10 Inserting Collection Tubes

Guide for loading collection tubes into the rack.

2.11 Changing from Standard Mode to Prep Mode

Steps to convert the Frac-950 between standard and prep operational modes.

2.12 Installing the Funnel-to-flask Kit

Instructions for installing the specific Funnel-to-flask accessory kit.

2.13 Changing from Prep Mode to Standard Mode

Steps to convert the Frac-950 from prep back to standard operational mode.

Operation

3.1 On/Off

How to power the Frac-950 on and off and understand indicator lights.

3.2 Operating Frac-950

Overview of controlling the Frac-950 via UNICORN software.

3.3 Collecting Fixed Fractions

Details on collecting fractions based on fixed volume or time intervals.

3.4 Collecting Peak Fractions

Instructions for collecting fractions based on detected peaks.

3.5 Feed Tube

Using the FeedTube instruction to advance the tube rack.

3.6 Ignore_LastTube

How to skip the last tube in a sequence when continuing collection.

3.7 Reset_Frac_Position

Resets the fraction collector to the first tube position.

3.8 AccumulatorWash

Procedure for manually washing the accumulator unit.

3.9 Setting Delay Volume

Configuring the delay volume in UNICORN for accurate fractionation.

3.10 Flow Control During Tube Change

Options for managing liquid flow during tube changes (Tube, DropSync, Accumulator).

3.11 Define Rack and Tube Parameters

Setting rack and tube parameters within a UNICORN method.

Maintenance

4.1 Periodic Maintenance

Information on the requirement for periodic maintenance.

4.2 Cleaning and Checking

Daily cleaning and inspection procedures for the unit.

4.3 Changing Capillaries

Guidance on when and how to replace the unit's capillaries.

4.4 Changing Waste Tubing

Guidance on replacing the waste tubing when it shows wear.

Trouble-shooting

5.1 Faults and Actions

Lists common faults and their suggested solutions.

Reference Information

A.1 Instrument

General description of the Frac-950 instrument and its operational modes.

A.2 Technical Specifications

Lists operating data, flow limits, and environmental requirements.

A.3 Tube Racks

Details on available rack designations, types, and tube combinations.

A.4 Physical Data

Specifications including degree of protection, materials, and dimensions.

A.5 Accessories and Consumables

Lists available accessories and consumable parts for the Frac-950.

Short Instructions

6.1 Manual Control Instructions

Quick checklist for manual control of fraction collection using UNICORN.