Applied Biosystems 7500 Fast Getting Started

Glossary
111
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
baseline-corrected
normalized reporter
(
∆ Rn)
The magnitude of normalized fluorescence generated by the reporter:
1. In experiments that contain data from real-time PCR, the magnitude of normalized
fluorescence generated by the reporter at each cycle during the PCR amplification. In
the
∆ Rn vs. Cycle amplification plot, ∆ Rn is calculated at each cycle as:
∆ Rn (cycle) = Rn (cycle) − Rn (baseline), where Rn = normalized reporter
2. In genotyping experiments and presence/absence experiments, the difference in
normalized fluorescence generated by the reporter between the pre-PCR read and the
post-PCR read. In the allelic discrimination plot (genotyping experiments) and the
presence/absence plot (presence/absence experiments),
∆ Rn is calculated as:
∆ Rn = Rn (post-PCR read) − Rn (pre-PCR read), where Rn = normalized reporter
See also normalized reporter (Rn).
biological replicates Reactions that contain identical components and volumes, but evaluate separate samples of
the same biological source (for example, samples from three different mice of the same
strain, or separate extractions of the same cell line or tissue sample).
See also technical replicates.
blocked IPC In presence/absence experiments, a reaction that contains IPC blocking agent, which blocks
amplification of the internal positive control (IPC). In the 7500/7500 Fast system software,
the task for the IPC target in wells that contain IPC blocking agent. See also negative
control-blocked IPC wells.
C
T
See threshold cycle (CT).
calibrator See reference sample.
chemistry See reagents.
comparative C
T
(
∆∆ C
T
) method
Method for determining relative target quantity in samples. With the comparative C
T
( ∆∆ C
T
)
method, the 7500/7500 Fast system software measures amplification of the target and of the
endogenous control in samples and in a reference sample. Measurements are normalized
using the endogenous control. The software determines the relative quantity of target in
each sample by comparing normalized target quantity in each sample to normalized target
quantity in the reference sample.
custom dye Dye that is not supplied by Applied Biosystems. Custom dyes may be adapted for use in
experiments on the 7500/7500 Fast system. When using custom dyes, the custom dye
should be added to the Dye Library and a custom dye calibration performed.
cycle threshold See threshold cycle (CT).
cycling stage In the thermal profile, a stage that is repeated. A cycling stage is also called an amplification
stage. For cycling stages, you can enable AutoDelta settings. See also amplification stage.

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English