Glossary
117
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
regression line
In standard curve and relative standard curve experiments, the best-fit line from the
standard curve. Regression line formula:
C
T
= m [log (Qty)] + b
where m is the slope, b is the y-intercept, and Qty is the standard quantity.
See also regression coefficients.
reject well
An action that the software performs during analysis to remove one or more wells from
further analysis if a specific flag is applied to the well. Rejected wells contain results
calculated up to the point of rejection.
relative standard
curve method
Method for determining relative target quantity in samples. With the relative standard curve
method, the 7500/7500 Fast system software measures amplification of the target and of the
endogenous control in samples, in a reference sample, and in a standard dilution series.
Measurements are normalized using the endogenous control. Data from the standard
dilution series are used to generate the standard curve. Using the standard curve, the
software interpolates target quantity in the samples and in the reference sample. The
software determines the relative quantity of target in each sample by comparing target
quantity in each sample to target quantity in the reference sample.
replicate group
A set of identical reactions in an experiment.
replicates
See technical replicates or biological replicates.
reporter
Fluorescent dye used to detect amplification. If you are using TaqMan
®
reagents, the
reporter dye is attached to the 5
′
end. If you are using SYBR
®
Green reagents, the reporter
dye is SYBR
®
Green dye.
reverse primer
An oligonucleotide that flanks the 3
′
end of the amplicon. The reverse primer and the
forward primer are used together in PCR reactions to amplify the target.
reverse
transcriptase
An enzyme that converts RNA to cDNA. Reverse transcriptase is added to the PCR reaction
to perform 1-step RT-PCR.
Rn
See normalized reporter (Rn).
ROX
™
dye
A dye supplied by Applied Biosystems and precalibrated on the 7500/7500 Fast systems.
ROX dye is used as the passive reference.
rs number
See refSNP ID.
run method
Definition of the reaction volume and the thermal profile for the 7500/7500 Fast instrument
run.
sample
The template that you are testing.
Sample DNA (10
✕
)
In the 7500/7500 Fast system software, a reaction component displayed on the Reaction
Mix Calculations tab of the Reaction Setup screen. The software assumes the sample DNA
is added to the reaction mix at a 10
✕
concentration. For example, if the reaction volume is
20
µ
L, the calculated volume of sample for 1 reaction is 2
µ
L.
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