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Applied Biosystems Veriti User Manual

Applied Biosystems Veriti
124 pages
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iv Veriti™ Thermal Cycler User Guide
Powering On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Setting Up the Veriti
Thermal Cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Recommended Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Setting Up Instrument Security and User Accounts . . . . . . . . . . . . . . . . . . . 2-9
Adding Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
Setting Other Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Chapter 3 Performing a Run
Selecting Disposables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Disposables for 96-Well Veriti
Thermal Cyclers . . . . . . . . . . . . . . . . . . . . . 3-2
Disposables for the 384-Well Veriti
Thermal Cycler . . . . . . . . . . . . . . . . . . 3-4
Disposables for the 60-Well Veriti
Thermal Cycler . . . . . . . . . . . . . . . . . . . 3-4
Loading Samples into the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Loading Samples in Veriti
96-Well Thermal Cyclers . . . . . . . . . . . . . . . . . . 3-5
Loading Samples in the Veriti
384-Well Thermal Cycler . . . . . . . . . . . . . . . 3-6
Loading Samples in the Veriti
60-Well Thermal Cycler . . . . . . . . . . . . . . . . 3-7
Creating a New Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Editing Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Performing a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18
Monitoring a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
Viewing and Printing the Run Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-25
Removing the Samples from the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-25
Chapter 4 Troubleshooting
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Returning an Instrument for Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Chapter 5 Utilities
Using GeneAmp
®
PCR System 9700 Run Methods . . . . . . . . . . . . . . . . . . . . . . 5-2
Upgrading System Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Calculating Melting Temperatures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
Viewing Instrument Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Restoring the Factory Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Running the Cycle Performance Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Tests Using the Temperature Verification Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Running the Heated Cover Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Running the Temperature Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-12
Running the Temperature Nonuniformity Test . . . . . . . . . . . . . . . . . . . . . . 5-13
TVK Recalibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-13

Table of Contents

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Applied Biosystems Veriti Specifications

General IconGeneral
BrandApplied Biosystems
ModelVeriti
CategoryDC Drives
LanguageEnglish