EasyManua.ls Logo

Beckman Coulter SW 41 Ti User Manual

Beckman Coulter SW 41 Ti
24 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
Page #1 background imageLoading...
Page #1 background image
L5-TB-047Q
November 2007
SW 41 Ti Rotor
Used In Beckman Coulter
Class H, R, and S
Preparative Ultracentrifuges
© 2007 Beckman Coulter, Inc.
Printed in the U.S.A.
Question and Answer IconNeed help?

Do you have a question about the Beckman Coulter SW 41 Ti and is the answer not in the manual?

Beckman Coulter SW 41 Ti Specifications

General IconGeneral
Rotor TypeSwinging Bucket
Maximum Speed41, 000 rpm
Number of Tubes6
Rotor MaterialTitanium
Tube Dimensions14 x 89 mm

Summary

Safety Notice

General Safety Guidelines and Precautions

Summary of safety information for rotor use, including handling hazards and operational safety.

Specifications

Preparation and Use

Prerun Safety Checks

Essential safety checks to perform before operating the rotor.

Rotor Preparation

Loading and Seating Buckets

Steps for loading tubes into buckets, greasing gaskets, and seating buckets.

Symmetrical Loading and Balance

Ensuring proper balance by symmetrical loading and matching liquid levels.

Operation

Rotor Installation

Procedure for correctly installing the rotor onto the ultracentrifuge drive hub.

Removal and Sample Recovery

Rotor Removal and Unloading

Steps for safely removing the rotor and unloading sample buckets.

Tubes and Accessories

Run Times

Calculating Pelleting Efficiency (k factor)

Formula and explanation for determining the rotor's k factor for run time calculations.

Run Speeds

Estimating Run Times

Using the k factor to estimate run times and adjust for different speeds.

Speed Reduction Circumstances

Conditions requiring a reduction in maximum run speed.

Selecting CsCl Gradients

CsCl Precipitation Avoidance

Guidance on limiting speed and density to prevent CsCl precipitation.

CsCl Gradient Curve Interpretation

Explanation of curves in Figures 2 and 3 for density and speed relationships.

Example B: Achieving Separation

Care and Maintenance

Cleaning Procedures

Decontamination

Handling Contaminated Materials

Guidelines for decontaminating rotors with toxic, radioactive, or pathogenic materials.

Sterilization and Disinfection