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ChemoMetec NucleoCounter NC-202 User Manual

ChemoMetec NucleoCounter NC-202
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NucleoCounter
®
NC-202
Instrument User Guide
Product No. 991-2020
Revision 1.8
27jan2023
ChemoMetec A/S
Gydevang 43 · DK-3450 Allerod · Denmark
Telephone: (+45) 48 13 10 20
Website: www.chemometec.com
E-mail: contact@chemometec.com

Table of Contents

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ChemoMetec NucleoCounter NC-202 Specifications

General IconGeneral
TypeAutomated Cell Counter
Measurement Time30 seconds
Viability MeasurementYes
Cell Size Range5-60 µm
Power Supply100-240 V, 50/60 Hz
Cell TypesMammalian cells, Yeast cells
StainingAcridine Orange and DAPI
InterfaceUSB, Ethernet
TechnologyFluorescence-based imaging

Summary

Introduction and Intended Use

System Overview and Purpose

Overview of the NucleoCounter NC-202 system and its intended use in life science.

Warnings and Precautions

Power Supply and Cables

Information on power supply, cables, USB connections, and safety precautions for the instrument.

Safety and Handling Precautions

Covers EMI, handling of consumables, and general biological safety measures.

Inspection, Unpacking and Installation

Installation and Operation Qualification

Details on the Installation Qualification (IQ) and Operational Qualification (OQ) protocols for the instrument.

Cell Counting with NucleoCounter NC-202

Instrument Overview

Description of the NucleoCounter NC-202 instrument's components and features.

Cassette Fixture and USB Connection

Details on the cassette fixture design and USB 3.0 connection for the instrument.

Via2-Cassette Loading and Handling

Instructions for loading and handling the Via2-Cassette for cell counting.

Cassette Calibration and Handling

Information on Via2-Cassette volume calibration and general handling best practices.

Instrument Controls

Interactive Controls

Details on the interactive controls of the NucleoCounter NC-202, including the RUN button and LED indicator.

Instrument Connections

Information about the power and USB connection ports on the NucleoCounter NC-202.

USB Connection and Initialization

Steps for connecting the instrument via USB and the initialization sequence.

Operation of NC-View Software and Instrument

NC-View Software Usage

Guide to using the NC-View software for data acquisition, image analysis, and result display.

Protocol Execution

Steps for executing a cell counting protocol using the instrument and software.

Piston Movements and Optical System Protection

Details on piston movements during analysis and protecting the instrument's optical system.

Maintenance and Cleaning

Cleaning Procedures

Procedures for cleaning the instrument cabinet, cassette fixture, and optical components.

Decontamination

Guidelines for complete decontamination of the NucleoCounter NC-202.

Troubleshooting

Firmware and Software Version

Instructions on how to find the firmware and software versions of the instrument.

Power-on Failure

Guidance on troubleshooting power-on failures, including checking the fuse and power supply.

Technical Specifications

Instrument Specifications

Detailed technical specifications of the NucleoCounter NC-202, including optics, power, and operating conditions.

Sample Media and Standards

Information on sample media requirements and relevant standards for the instrument.

Equipment and Accessories

Other Documentation

General Disclaimer

Appendix A: Changes from Latest Revision

Document Revision Details

Summary of changes, additions, and removals in the latest revision of the user guide.

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