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ChemoMetec NucleoCounter YC-100 User Manual

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NucleoCounter
YC-100
User's Guide
Revision 1.3
Technology that counts

Table of Contents

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ChemoMetec NucleoCounter YC-100 Specifications

General IconGeneral
BrandChemoMetec
ModelNucleoCounter YC-100
CategoryCash Counter
LanguageEnglish

Summary

Safety, Support, and Sales Information

Safety and Support Contacts

Precautions, technical support via email/phone, and sales information.

Disclaimers and Copyright Notices

Information subject to change; copyright and trademark information.

Warnings and Precautions for Operation

General Safety Guidelines

Precautions for operating the NucleoCounter, emphasizing manual consultation.

Cable and Connector Safety

Guidelines for USB and printer cables, connectors, and compliance.

Power Supply and Connection Details

Power Supply Requirements and Approved Models

Details on connecting the 12VDC supply and listing approved models.

Disconnecting Device and Mains Cord Compliance

Identification of disconnecting device and mains cord regulations.

Operational Safety and Handling Procedures

Power Supply Awareness and EMI

User awareness of power supply use and electromagnetic interference mitigation.

Consumable and Sample Handling Safety

Precautions for bottle-top dispensers, biological specimens, and pipetting.

General Handling and Sample Integrity

Pipetting Safety and Contamination Prevention

Avoid pipetting by mouth and prevent sample cross-contamination for accuracy.

1 Inspection and Unpacking of Equipment

Equipment Inspection and Reporting

Inspect for damage, report issues to carrier and ChemoMetec immediately.

Packing List Verification and Material Retention

Verify packing list and save materials for future reference.

2 Introduction to the NucleoCounter YC-100

System Overview and Advantages

Fast, objective, reliable, and safer alternative to manual cell counting.

System Components and Technology

NucleoCassette, instrument, lysis buffer, and fluorescence microscopy.

2.1 Fluorescence Microscope Operation

Microscope Components and Measurement Activation

Details components and how measurement is activated.

Light Path and Detection Principles

PI excitation by green light and red fluorescence detection.

2.1.2 Built-in Image Analysis

Image Analysis Process and Result Calculation

Software analyzes images, counts nuclei, and calculates cell density.

2.2 The NucleoCassette

NucleoCassette Design and Functionality

Disposable device with piston, flow system, and measurement chamber.

2.2.1 NucleoCassette Loading Procedure

Cassette Loading and Sample Introduction

Step-by-step guide to loading the cassette with lyzate mixture.

2.2.2 Fluorescent Dye and Sample Volume

Propidium Iodide Function and Analysis Volume

PI's role in staining and the analyzed sample volume.

2.2.4 Cassette Handling and 2.3 Lysis Buffer

NucleoCassette Handling Precautions

Avoid touching the measurement window to prevent contamination.

Lysis Buffer (Reagent Y100) Usage

Reagent Y100 for lysing yeast cells; special preparation may be needed.

3 Control Interface Elements

3.1 Interactive Controls and Buttons

Details on the instrument's control buttons (On/Off, Run, Esc, Del).

3.1.2 Keypad Functionality

Description of the numeric and function keys for data entry.

3.2 Instrument Display System

Status Indicator Light Functionality

Explains the color coding of the indicator light for instrument status.

Display Screen Information Content

Communicates unit ID, software version, operating status, and results.

4 Installation and Start-up Procedures

4.1 Power On and Off Sequence

Connecting the power supply and turning the instrument on/off.

Instrument Start-up Process and Warnings

Instrument's self-check during start-up and associated warnings.

4.1.2 Shutting Down the NucleoCounter

Instrument Shutdown Display

Procedure for turning off the NucleoCounter and its display.

4.2 NucleoView Software Installation

Software Installation Reference Guide

Refers to the NucleoView User's Guide for installation.

5 Operation of the NucleoCounter YC-100

5.1 Ready Mode Indication

Indicates the instrument is ready for cell counting.

5.2 Cassette Insertion and Removal

Procedure for inserting and removing the NucleoCassette.

5.2.1 Optical System Protection Measures

Protecting the optical system by keeping the sample compartment lid closed.

5.3 Measurement Initiation with Run Button

Actuator Movement During Measurement

Describes actuator movement and sample transport during analysis.

5.3.2 Result Presentation and Analysis Time

How results are recorded, analyzed, and displayed within 30 seconds.

5.3.3 External Computer Connection for Results

Viewing Results with NucleoView Software

Connecting to a PC for viewing results and images via NucleoView.

5.4 Result Display Options

5.4.1 Result Mode Selection and Formatting

Explains and demonstrates how to select different result display modes.

5.4 Parameter Settings and Adjustments

Saving and Cancelling Parameter Changes

How to save or cancel parameter changes upon instrument shutdown.

Date, Time, and Counter Reset

Setting date and time, and resetting the analysis counter.

LCD Contrast Adjustment

Using keys to adjust LCD contrast, with default value and range.

5.4.6 Instrument Information Display

Saving LCD Contrast Settings and Instrument Information

Confirming new LCD contrast value and displaying instrument details.

5.5 Instrument Sensitivity Adjustment

Sensitivity Settings Dialogue and Selection

Dialogue for selecting sensitivity settings like Haploid, Diploid, Small.

5.6 Sensitivity Settings and Zero Count Check

Sensitivity Setting Selection and Optimization

Labels are guidelines; use NucleoView for optimal settings.

Effect of Sensitivity Settings on Results

Inappropriate settings can cause over/underestimation of cell concentration.

Performing the Zero Count Check

Procedure to perform a Zero Count Check for contamination detection.

Zero Count Check Procedure Steps

Initiating the Zero Count Check

How to initiate the check by typing 'F50 + ل'.

Zero Count Check Prompts and Interaction

Prompts for removing cassette and closing the lid during the check.

Zero Count Check Results and Error Handling

Interpreting Zero Count Check Results

Display indicates if the check passed (OK) or failed (ERROR).

Actions for Zero Count Check Errors

Cleaning the insertion slit and re-running the check for errors.

6 Total and Viability Count Methods

6.1 Non-viable Cell Concentration Determination

Method for determining non-viable cells using dye permeability.

6.2 Total Cell Concentration Measurement

Procedure for determining total cell concentration via cell lysis.

6.3 Determination of Cell Viability

Viability Calculation Formula and Variables

Formula for calculating cell viability and definition of variables.

7 Maintenance of NucleoCounter YC-100

7.1 Instrument Cleaning Procedures

General cleaning recommendations for maintaining instrument performance.

7.1.1 Cassette Insertion Area and 7.1.2 Optical Elements Cleaning

Cleaning the insertion area and optical parts for optimal image quality.

7.1.3 Dust Particle Removal

Dust Removal from Insertion Slit

Using compressed air to remove dust from the insertion slit.

7.1.4 Spill of Liquid Handling

Handling Liquid Spills on the Instrument

Guidance for liquid spills and contamination; consult manufacturer.

8 Troubleshooting - Error Messages

8.1 No Valid Cassette Error

Error message when the instrument does not recognize the cassette.

8.2 Analysis Aborted Error

Error message for aborted analysis due to flow sensor or timing issues.

8.3 Actuator Errors, 8.4 Sample Analysis Errors, and 8.5 Sensor Errors

Actuator Error Handling and Troubleshooting

Error messages related to actuator movement and troubleshooting steps.

Sample Analysis Error Message

Error message when cell concentration determination fails.

Sensor Error Detection and Implications

Error message for faulty sensors and its impact on accuracy.

8.6 Power-On Failure Troubleshooting

Power Supply and Fuse Checks

Verifying power supply connection and inspecting/replacing fuses.

Fuse Holder Location and Replacement

Shows fuse holder location and specifies fuse replacement type.

9 Technical Specifications

9.1 NucleoCounter YC-100 Specifications

Detailed technical specifications covering specificity, sample types, and operation.

Physical Data, Power, and Interfaces

Instrument dimensions, power requirements, operation conditions, and interfaces.

Environmental, EMC/EMI, and Cassette Specifications

Environmental and EMC/EMI Standards

Installation ratings, environmental conditions, and EMC/EMI compliance.

9.2 NucleoCassette Specifications

Details on NucleoCassette reagent content, storage, and stability.

10 Equipment and Accessories

10.1 Equipment and Accessories List

Comprehensive list of items and their part numbers for the system.

10.2 Power Supply and Accessories

Approved External Power Supplies

Specific models of Class I and Class II power supplies.

Mains Power Cord Sets Information

Information on detachable power supply cord sets for power supplies.

Additional Accessories and Application Notes

Lists printers, paper, ribbon supplies, and reference application notes.

Mains Power Cord Specifications and Compressed Air

Power Cord Set Specifications

Specifications for Class I and Class II power cord sets for US and Europe.

10.4 Dry Compressed Air for Cleaning

Use of compressed air for dust removal from the optical system.

10.5 External Printer (Optional)

Connecting Optional Printers for Results

Instructions for connecting impact or thermo printers for test results.

Printer Output Port Location and Connection

Shows port location and illustrates connecting the printer cable.

11 Appendix 1: WEEE Directive Information

WEEE Disposal Information for Various European Countries

WEEE disposal instructions for UK, Sweden, Slovakia, Portugal, Poland, and France.

WEEE Disposal Information by Country (Continued)

WEEE Disposal Information for Norway, Netherlands, Latvia, Lithuania, Italy, and Hungary

WEEE disposal instructions for Norway, Netherlands, Latvia, Lithuania, Italy, and Hungary.

WEEE Disposal Information for Greece, Germany, Denmark, and Czech Republic

WEEE disposal instructions for Greece, Germany, Denmark, and Czech Republic.

WEEE Disposal Information by Country (Continued)

WEEE Disposal Information for Finland, Estonia, Spain

WEEE disposal instructions for Finland, Estonia, and Spain.

Disclaimer on Data Accuracy and Validation

Data Accuracy, Potential Deviations, and Validation Recommendations

Information accuracy, potential deviations, and recommended validation against reference methods.