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CoolLED pE-300 Series User Manual

CoolLED pE-300 Series
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DOC-005 Iss 15 1
User Manual
pE-300 Series
White Light Fluorescence Illumination System

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CoolLED pE-300 Series Specifications

General IconGeneral
BrandCoolLED
ModelpE-300 Series
CategoryLaboratory Equipment
LanguageEnglish

Summary

Introduction to CoolLED pE-300 Series

Safety Precautions for Illumination System

UV Light Exposure and Skin Protection

Avoid eye and skin exposure to UV light; never look directly into the light output beam.

Secure Attachment and Power Connection

Ensure secure attachment to microscope before powering on; use supplied power cords.

Operation Without Microscope Attachment

When not attached, personnel must wear eye shielding and protective clothing.

Power Supply and Cable Safety

Use only supplied power supply and cord; plug in cable only after attachment.

No User-Serviceable Parts

The Light Source contains no user-serviceable parts; tampering voids safety.

Compliance with Safety Standards

Product conforms to EN/IEC 61010-1 and EN62471 (Photo-biological Safety).

Cleaning and Maintenance Safety

Clean exterior with damp cloth; isolate DC power supply before cleaning optics.

EMC Compliance

Product tested to IEC/EN 61326-1 for electromagnetic compatibility (Class B).

PE-300 Series Variants

pE-300white Model

Offers individual control of three channels via Control Pod, USB, or TTL.

pE-300lite Model

The simplest model with global intensity control via the manual Control Pod.

pE-300ultra Model

Provides the greatest control with multi-channel sequencing via TTL and inline filters.

Getting Started: System Components

Installation and Setup

Unpacking and Component Check

Carefully unpack all components and check for any missing or damaged items.

Connecting the Control Pod

Instructions for connecting the Control Pod cable to the LED Light Source using alignment guides.

Connecting the DC Power Supply

Connect the DC power supply connector, ensuring it's the supplied unit.

Attaching the LED Light Source

Ensuring Proper Airflow

Maintain a 200 mm gap on sides for adequate airflow to the cooling system.

Connecting Mains Power

Connect the mains lead to a socket and the IEC connector to the power supply.

LED Configuration for White Light

pE-300 Series Spectra

Displays spectral output graphs (SB and MB) showing relative irradiance vs. wavelength.

Channel Control and Configuration

Independent Channel Control

User control over three main emission peaks (UV, Blue, GYR) for pE-300white/ultra.

Violet Spectrum Variant

A variant shifting the first peak from UV to violet for multi-band filter sets.

pE-300lite Configuration Options

Choice between SB and MB configurations with global intensity control for pE-300lite.

Manual Control Operation

On/Off Control

How to switch LEDs on and off using the dedicated 'on/off' button on the Control Pod.

Start-up and Settings Persistence

The system reverts to the last powered-down settings upon startup.

Intensity Adjustment

Controlling individual LED intensities to balance different stains for optimal imaging.

Individual Channel Selection

Switching individual bands on or off (de-selecting) using the 'select' button.

Remote TTL Operation

Global TTL Triggering

Control on/off function globally via a TTL signal to the BNC socket.

Individual Channel TTL Triggering (pE-300ultra)

Control individual channels using three additional BNC sockets on the pE-300ultra.

Sequence Runner Mode (pE-300ultra)

Sequential triggering of multiple channels using a single TTL signal via the Sequence Runner feature.

TTL Triggering Information

Technical details on TTL input specifications and LED switching speeds.

Remote USB Operation

USB Interface and Connection

Connecting the Light Source to a computer using a USB cable for remote control.

Driver Installation

Steps for installing driver files to allow the system to be recognized by Windows.

COM Port Assignment

Checking assigned COM ports in Device Manager for software connectivity.

Optical Setup Procedures

Direct Fit Optical Adjustment

Performing a one-time adjustment for optimal brightness and field of view.

Liquid Light Guide Setup

Instructions for inserting the light guide, managing bend radius, and using the cradle.

Additional Filtering (pE-300ultra)

Excitation Filter Holder Slides

Details on the filter holder slides, their capacity, and installation orientation.

Control Pod Settings and Information

Display Backlight and Contrast Settings

How to adjust the backlight and contrast of the Control Pod display for different models.

System Information Display

Accessing hardware and firmware revisions, model, and serial number via the Control Pod.

LED Usage Monitoring

Retrieving and viewing the total operational time recorded for the LEDs on the system.

Routine Care and Maintenance

Low Maintenance Requirements

The system requires minimal maintenance and has no field-serviceable parts.

External Surface Cleaning

How to clean the exterior using a damp cloth, avoiding optical surfaces.

Optical Surface Cleaning

Procedures for cleaning optical surfaces, using air dusters and standard lens cleaning.

Fitting to Different Microscopes

Microscope Adaptor System

How to fit the system using compatible adaptors and the process of changing them.

Special Microscope Requirements

Notes microscopes needing special optics or settings, requiring specific light sources.

Product Specifications

Power Requirements and Consumption

Specifies voltage, current, and power consumption across different operating modes.

Dimensions and Weight

Provides physical dimensions and weight for the Light Source, Control Pod, and Power Supply.

Environmental Operating Conditions

Defines the acceptable operating temperature range for the illumination system.

Product Options and Support

Product Options and Order Codes

Directs users to the website for full details on available product options and order codes.

Warranty and Repair Procedures

Guidelines for warranty claims, fault reporting, and contacting customer support.

Compliance and Environmental Information

Refers to website for compliance statements and environmental responsibility, including recycling.

CoolLED Recycling Program

Details the program for returning used light sources for free recycling.

Contact Details

Appendix 1: Advanced Features

Spectral Output and LED Channels

Presents spectral graphs (MB/SB) and explains the UV-Violet, Blue, and GYR LED channels.

Benefits of Individual Channel Control

Highlights advantages like fast control, sustainability, contrast enhancement, and reduced photobleaching.

High-Speed Imaging Capabilities

Explains how high-speed imaging is achieved by eliminating filter wheels and using TTL triggering.

Product Overviews: pE-300ultra and pE-300white

Summarizes key features of the pE-300ultra and pE-300white illumination systems.