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General| Number of Lasers | Up to 5 |
|---|---|
| Number of Detectors | Up to 64 |
| Power Requirements | 100-240 VAC, 50/60 Hz |
| Type | Flow Cytometer |
| Laser Configuration | 405 nm, 488 nm, 561 nm, 640 nm |
| Sample Type | Cells |
| Software | SpectroFlo |
| Application | Immunophenotyping, cell cycle analysis, apoptosis |
| Cell Size Range | 1-50 μm |
| Data Output | FCS 3.1 |
| Weight | Approximately 150 kg (varies by configuration) |
Compliance and warnings related to FCC regulations.
Overview of the manual's content and purpose.
Essential safety precautions, symbols, and guidelines.
Contact information and resources for instrument support.
Description of the Aurora cytometer and workstation system.
Front panel, fluidics system, and flow cell overview.
Optical schematic and SpectroFlo software modules.
Principles of spectral unmixing and reference spectra.
Procedures for filling sheath and emptying waste tanks.
Step-by-step guide for powering on the cytometer.
Instructions for safely powering off the instrument.
Performing and understanding Daily QC and reports.
Creating, acquiring, and updating reference controls.
Levey-Jennings tracking, gain settings, and alarm ranges.
Raw vs unmixed data, live and post-acquisition unmixing.
Steps for experiment setup, acquisition controls, and worksheets.
Creating and customizing plots, gates, and statistic displays.
Live and post-acquisition unmixing procedures.
Performing unmixing and compensation using FCS files.
Using virtual filters to simulate conventional compensation.
Managing QC beads, fluorescent tags, labels, and templates.
Customizing acquisition, worksheet, plot, and other settings.
Adding, editing, and managing user accounts.
Overview of scheduled and unscheduled maintenance tasks.
Cleaning SIT, sheath filter, and flow cell.
Long Clean procedure for fluidic system.
Replacing sheath filter and SIT tubing assembly.
Resolving air in sheath filter and no events displayed.
Troubleshooting low event rate, distorted data, and high CVs.
Technical specifications of optical components and lasers.
Specifications for fluidics and fluorescence detection capabilities.
Hardware specs for workstation and installation criteria.
List of tanks, lids, caps, and filters with part numbers.
List of sample lines, QC beads, and bypass tubing.
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