Biometra TRIO Function and setup
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3 Function and setup
3.1 Field of Applications
Developed in 1983 by Kary Mullis PCR (Polymerase Chain Reaction) is now a common
and often indispensable technique used in medical and biological research labs for a
variety of applications. The Biometra TRIO is an end-point Thermal Cycler and intend-
ed to amplify nucleic acids by repeated cycles of heating and cooling using DNA-
Polymerases in a PCR reaction. The development of the instrument had the goal to
combine latest technology with stylish design and user-friendly software. The user
interface consists of a touchscreen with a graphical or tabular display that shows the
time, status, and temperature profile for each run. A touchscreen keypad allows you to
enter information into fields on the screen. The Biometra TRIO can run three inde-
pendent PCR reactions simultaneously and is especially helpful when versatile samples
are to be incubated using different PCR programs.
3.2 General Description
There are three models of the Biometra TRIO Thermal Cycler:
Biometra TRIO 48 for 0.2 ml tubes, plates or strips
Biometra TRIO 30 for 0.5 ml tubes
Biometra TRIO combi for 0.2 ml tubes, plates or strips or 0.5 ml tubes
The instrument is controlled by a 7’’ touchscreen display and easy to use interface. PCR
protocols and Run-Logfiles can be saved to a connected USB drive. By using the USB
functions PCR protocols can be exchanged easily between instruments and Run-
Logfiles can be stored for documentation reasons on PC.
Figure 1 Biometra TRIO Thermal Cycler