Protocol Wizard Biometra TRIO
66 Version 03_19
The next screen lists the all steps of the program that will be created by the software:
Figure 44 Biometra TRIO protocol wizard view parameter screen
Press the [CREATE PROGRAM] button to enter the Biometra TRIO programming screen
(see chapter "Creating, editing and saving programs" p. 39).
In the programming screen the program is displayed in graphical or spreadsheet mode
and can be edited. To save the program created by the protocol wizard follow the in-
structions in chapter 7.9.
Note: Programs have to be saved before they can be started. If the program has been
created but should not be saved confirm the following security query to discard the
program:
Figure 45 Biometra TRIO security query
10.5 Primer Annealing Temperature Calculator
Based on the primer sequences the Biometra TRIO protocol wizard can calculate the
theoretical primer annealing temperature (T
a
). Press the [CALC TA ] button in the Bi-
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