Endress+Hauser Analytik Jena Biometra TRIO - Fig. 22 Biometra TRIO Edit Step Screen

Biometra TRIO Creating, editing and saving programs
49
Time increment (e.g. for long range PCR). Increases the set time
with each cycle by the defined increment. The increment can be
set from 0s to 240s.
See section "Set time incre-
ment" p. 51
Average ramp rate between steps. The average ramp rate can be
set from 0.1°C/s to max in tenths of a degree (The maximum
ramp rate is sample block specific). The ramp rate specifies the
speed at which the selected step is reached with.
See section "Adjust ramp rate"
p. 51
Temperature Optimisation Step (TOS). For optimisation of new
PCR protocols very often gradient enabled Thermocyclers are
used. The Biometra TRIO offers no gradient function but by the
temperature optimisation step (TOS) function pograms making
use of the three blocks and delivering different annealing temper-
atures at the defined step can be created.
It is possible that in some models this display may show a slightly
higher maximum ramping rate than is achieved according to the
technical specifications of the device. This is technically justified
and achieves optimum control of the maximum value. In fact, the
maximum heating and cooling rate is achieved as specified in the
technical specifications.
See section "Edit parameter or
step" p. 52
Fig. 22 Biometra TRIO edit step screen
Note: To program a step at least the temperature and the hold time must be defined.
To set the temperature touch the corresponding input box and enter a value between
3.0°C and 99.0°C. Use the Tab button or touch the corresponding input box to activate
the next input box and enter the time in h:mm:ss.
The Biometra TRIO software offers to program several steps subsequently. Use the cur-
sor buttons to navigate between the steps.
Previous step