Endress+Hauser Analytik Jena Biometra TRIO - Set Cycles; Fig. 23 PCR Program with Loop

Creating, editing and saving programs Biometra TRIO
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Next step
The currently activated step is high-lighted in the program overview table (see Fig. 22).
Note: By navigating between steps it is not necessary to confirm the settings for each
single step and to leave the edit step screen but you can fill in the parameters for several
steps and confirm the settings after all steps are programmed.
7.6.1 Set cycles
A typical PCR program consists of repeated steps for denaturation, annealing and exten-
sion. For the repetition of steps the Biometra TRIO software offers to program loops. For
a cycle two parameters have to be set:
1. The step number to go back to (Goto)
2. The number of repeats (Cycles)
To program a loop select the last step of the cycle and enter the step number the pro-
gram shall go back to in the input box Goto (see Fig. 22). To define the number of re-
peats, enter a number in the input box Cycles.
For example to program the cycles for the following protocol select step 4, enter 2 in the
input box Goto and enter 35 in the input box Cycles.
Fig. 23 PCR program with loop
In this example the instrument first executes step 1 to step 4, then repeats step 2 to
step 4 for 34 times, performs the final extension step at 72°C and holds the temperature
indefinitely at 16°C.
Note: The Biometra TRIO allows to set maximal two interlaced loops. It is possible to
program a loop within a loop but it is not allowed to set loop in loop in a loop.