Endress+Hauser Analytik Jena Biometra TRIO - Set Temperature Increment; Set Time Increment; Adjust Ramp Rate

Biometra TRIO Creating, editing and saving programs
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7.6.2 Set temperature increment
For some applications, like for example touch down PCR, temperature increments are
used. The temperature is increased or decreased with each cycle by the set value. To
program a temperature increment press the following button (see Fig. 22) and fill in a
value between +20°C and -20°C in the corresponding input box.
Temperature Increment (ΔT)
Note: Temperature increments only become active in repeated steps (cycles). If a tem-
perature increment has been programmed, the small symbol will be shown at the
corresponding step in the graphical programming mode.
7.6.3 Set time increment
For some applications, like for example long range PCR, time increments are used. The
time is increased with each cycle by the set value. To program a time increment press
the following button (see Fig. 22) and fill in a value between 0s and 240s in the corre-
sponding input box.
Time Increment (Δt)
Note: Time increments only become active in repeated steps (cycles). If a time incre-
ment has been programmed, the small symbol will be shown at the corresponding
step in the graphical programming mode.
7.6.4 Adjust ramp rate
The average ramp rate can be adjusted for each step. The ramp rate adjustment can be
useful if PCR programs are transferred from slower Thermal Cyclers the Biometra TRIO
or for special PCR applications like for example telomerase PCR. The average ramp rate
specifies the speed at which the selected step is reached with. To adjust the ramp rate
press the rampe rate button.
Ramp Rate (ΔR)
Then enter a value between 0.1°C/s and max. in tenths of degree Celsius.
It is also possible that the entered ramp rate is applied to all steps in the PCR program.
For this the checkbox Apply Rate to all steps must be activated.
Note: The maximum average rate depends on the sample block installed in the Bi-
ometra TRIO Thermal Cycler. If the ramp rate has been adjusted, the small symbol
will be shown at the corresponding step in the graphical programming mode.