Endress+Hauser Analytik Jena Biometra TRIO - Set Temperature Optimisation Step (TOS); Insert Step; Fig. 24 Biometra TRIO Temperature Optimisation Step Screen

Creating, editing and saving programs Biometra TRIO
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7.6.5 Set Temperature Optimisation Step (TOS)
For optimisation of new PCR protocols very often gradient enabled Thermocyclers are
used. The Biometra TRIO offers no gradient function but by the temperature optimisa-
tion step (TOS) function programs making use of the three blocks and delivering differ-
ent annealing temperatures at the defined step can be created.
Sample replicates are distributed across the sample blocks and are tested for the tem-
perature giving the optimal result. To program a Temperature Optimisation Step press
the following button.
Temperature Optimisation Step (TOS)
Enter the desired annealing temperature (Annealing Temp.) and define a temperature
increment (Increment) between the sample blocks. The effective temperatures for all
sample blocks are displayed (see Fig. 23).
Note: The annealing temperature is set for sample block 2 and the increment defines
the temperatures for sample block 1 and 3. When setting a positive increment the tem-
perature in sample block 1 is the lowest and the temperature in sample block 3 is the
highest. Use the minus button on the keypad to enter a negative increment. When a
negative value is used as increment the temperature in sample block 1 is the lowest and
the temperature in sample block 3 is the highest.
Fig. 24 Biometra TRIO Temperature Optimisation Step screen
7.7 Insert step
To insert a new step, select a program step in the Biometra TRIO programming screen
(see Fig. 16 and Fig. 17) and press the insert step button.