Biometra TRIO Protocol Wizard
69
New Polymerase
Edit Polymerase
In the next screen (see Fig. 40) touch the corresponding input boxes to enter a name for
the polymerase and set parameters for all requested program steps. With two excep-
tions for most program steps the temperature and the holdtime must be defined:
The temperature for the annealing step depends on the calculated primer melting
temperature (T
m
-value) and has to be set individually for each primer pair (see sec-
tion "Protocol Wizard parameter screen" p. 71).
The holdtime for the elongation step becomes calculated by the expected product
length (see section "Primer Annealing Temperature Calculator" p. 73).
The corresponding input boxes for the annealing temperature and elongation time are
shown in grey and cannot be activated.
Fig. 40 Biometra TRIO screen for editing program templates for polymerases
After creating the program template for a new polymerase or editing the program tem-
plate of a polymerase press:
Done
In the next screen (see Fig. 41) touch the corresponding button to select a storage place
for the program template (polymerase). If the number of polymerases is higher than can
be maximal displayed in the list, the buttons above and below the polymerase list can be
used to scroll up and down.
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