Endress+Hauser Analytik Jena Biometra TRIO - Fig. 47 Biometra TRIO Screen for Primer Concentration Adjustment

Protocol Wizard Biometra TRIO
74
Fig. 47 Biometra TRIO screen for primer concentration adjustment
Press the edit primer button to toggle to the primer annealing temperature calculator
screen (see Fig. 47).
Edit primer
Or press the calc. primer button to calculate the theoretical primer annealing tempera-
ture (T
a
).
Calc. primer
The calculated values for the forward primer melting temperature (T
m
), reverse primer
melting temperature (T
m
), mean primer melting temperature (T
m
) and calculated an-
nealing temperature (T
a
) are displayed.
Press the save temp. button to transfer the calculated primer annealing temperature to
the protocol wizard (see Fig. 46).
Save temp.
The calculated T
a
value will be displayed in the input box for the primer annealing tem-
perature.
Press the info button to get information on the algorithms used for annealing tempera-
ture calculations.
Info
For short sequences of 14 nucleotides or less the software calculates the primer anneal-
ing temperature (T
a
) based on the primer T
m
values as follows: