ActionStep
Cut the waste tubing from the Fraction collector and the Buffer tray to ap-
propriate length. It is important that the tubing is not bent and will not be
submerged in liquid during the run.
3
Note:
If the tubing is too short, replace it with new tubing. Do not lengthen the tubing
as this might cause obstruction of the tubing and flooding in the Frac chamber.
CAUTION
Make sure that the waste vessels will hold all the produced volume
of the run. For ÄKTA avant 25, a suitable waste vessel should typi-
cally have a volume of 2 to 10 liters. For ÄKTA avant 150, a waste
vessel should have a volume of 40 liters.
Plug unused valve ports
It is recommended to plug all unused valve ports with stop plugs before starting a run.
See Section 9.3 Tubing and connectors, on page 432 for information about connectors.
ÄKTA avant User Manual 29035184 AE 161
5 Operation
5.2 Prepare the flow path
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