ActionStep
Connect a 25 to 30 ml syringe to the left purge valve of the selected system
pump. Make sure that the syringe fits tightly into the purge connector.
4
Open the purge valve by turning it counterclockwise about 3 quarters of a
turn. Draw 10 ml of liquid slowly into the syringe with a rate of about 1 ml
per second.
5
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents.
6
Repeat steps 3 to 5 for the other three purge valves of the system pumps
to get rid of air in all pump heads. Keep the system flow running during this
procedure.
7
Check that there is no air left in the pump by following the instructions in
Validate prime or purge of System Pump A or B or Sample Pump, on page172.
8
End the run
Click the End button in the System Control toolbar to end the run.
ÄKTA avant User Manual 29035184 AE 181
5 Operation
5.4 Prime inlets and purge pump heads
5.4.3 Prime Q inlets
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