GE AKTA avant - Sample Application

5.7 Sample application
Introduction
This section describes the different sample application techniques that can be used with
ÄKTA avant. The table below shows the alternatives for sample application available in
the Sample application phase of a method.
Compatible loopsViaSample applica-
tion
•
Not applicable
•
Sample pump
•
Sample pump and air
sensor
Inject sample di-
rectly onto column
•
Sample loop
•
Superloop, 10 ml
•
Superloop, 50 ml
•
Superloop, 150 ml
•
Syringe
•
Sample pump
Inject sample from
loop
In order to avoid sample carry-over when switching techniques for loading
samples, wash the valve with buffer between the loading of two different
samples. For example, when switching from loading sample in the loop to
loading sample directly onto the column with the valve in Direct inject position.
Note:
When using a pump for sample application, it is important to prime inlets and purge the
pump before using the pump to load the sample. See further instructions in Section 5.4.2
Prime sample inlets and purge Sample Pump, on page 173.
When loading sample using an external air sensor, the sensor should be installed accord-
ing to Adapter for air sensor, on page 49.
ÄKTA avant User Manual 29035184 AE 189
5 Operation
5.7 Sample application