Maximize precision and accuracy
To achieve full precision and accuracy when a volume of sample is injected directly onto
the column, make the following selections in the Sample Application phase of the method
to be run (refer to Section 5.9 Create a method and perform a run, on page 225 for more
information on methods and phases):
•
select Inject sample directly onto the column,
•
select Inject fixed sample volume and set the volume to be injected,
•
enable the function Prime sample inlet with and set the volume to be used for
priming.
Result: the step Finalize sample injection is automatically deactivated in order to
maximize precision and accuracy. See Minimize sample loss, on page 191 for more
information.
If manual priming of the flow path up to the injection valve is preferred,
enable Prime sample inlet with but set the volume to 0 ml.
Note:
ÄKTA avant User Manual 29035184 AE 193
5 Operation
5.7 Sample application
5.7.1 Sample application using direct injection into the column
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