GE AKTA avant - Page 433

Tubing types
The table below shows the tubing types used in ÄKTA avant.
Volume/cmScope of useColorDescription
0.5 μlHigh pressure tubing
Reference capillary 1
BluePEEK, o.d. 1/16", i.d. 0.25
mm
2.0 μlHigh pressure tubingOrangePEEK, o.d. 1/16", i.d. 0.50
mm
4.4 μlHigh pressure tubingGreenPEEK, o.d. 1/16", i.d. 0.75
mm
7.8 μlHigh pressure tubingBeigePEEK, o.d. 1/16", i.d. 1.0
mm
20.0 μlInlet tubingTranspar-
ent
FEP, o.d. 1/8", i.d. 1.6
mm
66.0 μlInlet tubing for high flow
rate and high viscosity
Transpar-
ent
FEP, o.d. 3/16", i.d. 2.9
mm
4.4 μlNarrow inlet tubing (op-
tional)
Transpar-
ent
ETFE, o.d. 1/16", i.d. 0.75
mm
7.8 μlOutlet and waste tubingTranspar-
ent
ETFE, o.d. 1/16", i.d. 1.0
mm
35.0 μlPump rinse solution
tubing
Transpar-
ent
Silicone, o.d. 4.1 mm, i.d.
2.1 mm
0.5 mlWaste tubing from
Buffer tray
Transpar-
ent
Silicone, o.d. 12 mm, i.d.
8 mm
Many different sizes/types of tubing can be connected to a chromatography
system. Tubing with a smaller inner diameter (i.d.) holds less delay volume and
will therefore generate less dilution of the protein peak. Narrow tubing, how-
ever, increases the system pressure, especially when running at high flow
rates. The tubing used should match the application needs.
Note:
Tubing connectors
The table below shows the tubing connectors used in ÄKTA avant.
Use with tubing...Description
PEEK and ETFE with o.d. 1/16"Fingertight connector, 1/16"
ÄKTA avant User Manual 29035184 AE 433
9 Reference information
9.3 Tubing and connectors