ActionStep
Insert the end of the sample inlet tubing into the sample container.
Tip:
If the tubing is filled with buffer, introduce a small air bubble into the sample
tubing between the buffer and the sample. This will mark the progress of the
sample front along the flow path tubing.
2
Set a suitable pump flow rate, for example 100 mL/min. Follow the sample
front in the tubing. (The sample front may be difficult to follow if the flow
rate is too high.)
3
When the sample front has passed through the inlet valve, click Pause .
4
Switch the inlet valve to buffer inlet.5
Click Continue , change the flow rate to maximum (600 mL/min), and
wait until all air bubbles have been removed through the Waste 2 outlet on
the column valve.
6
Result: The flow path contains sample up to the inlet valve and buffer from
the inlet valve to the column valve.
Click End .
7
ÄKTA pilot 600 User Manual 29274559 AA 67
4 Operation
4.1 Sample application
4.1.2 Apply sample from a system inlet
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