Editing the method
A method created with the method wizard can be later edited in the Method Editor via,
for instance, the Run Setup dialog box. Open the dialog box by clicking Run Setup on
the View menu.
Variable value can be changed by clicking the Variables tab and changing the value of
CV_Equilibration.
The default values of variables are:
•
Flow velocity: 100 cm/h.
•
Equilibration volume: 1.30 column volumes.
•
Tracer volume loaded on the column: 2.5% of the column volume.
For the method to work correctly, changing of the flow velocity requires
changing of the tracer volume and vice versa.
Note:
Only advanced users should change variables, shown in the detailed section
(only displayed when Show details is checked).
Note:
ÄKTA ready Operating Instructions 28960345 AD 169
10 Creating a method
10.3 Creating a column efficiency test
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