GE Amersham Imager 680 - Fluorescence Capture

5.5.4 Fluorescence capture
Introduction
Fluorescence image capturing is performed with Epi-illumination (Blue, Green and Red)
or Trans-UV illumination. The Epi-illumination method allows for both single and multi-
channel detection. Fluorescence capturing is performed using a light source and emission
filter.
Fluorescence capture is only available with Lower tray position.
Note:
The methods available in Amersham Imager 680 configurations are described in the
following table:
680 RGB680 QC680 UV680
xEpi-RGB
xCustom
xxxTrans-UV
The Custom method allows for different combinations of the blue, green or
red light sources and an emission filter of choice.
Note:
Fluorescence capture options
The table below illustrates sample types for fluorescence capturing:
Light source (location in-
side)
Tray and accessory
(placement only in the
lower tray position)
Sample type
Epi-Blue, Green, and Red
fluorescence (top)
Black TrayGel (e.g. Cy™2/Cy3/Cy5)
Epi-Blue, Green, and Red
fluorescence (top)
Black TrayMembrane (e.g. ECL Plex™
Cy2, ECL Plex Cy3, ECL
Plex Cy5)
Trans-UV (bottom)UV Trans Tray (transpar-
ent) + gel sheet
Gel (e.g. EtBr/SYBR™
Green)
Amersham Imager 680 Operating Instructions 29271308 AB 141
5 Operation
5.5 Capture an image
5.5.4 Fluorescence capture