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FluoroMax-4
Horiba Scientific FluoroMax-4 User Manual
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Table of Contents
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Table of Contents
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0: Introduction
9
About the Fluoromax ® -4 and Fluoromax ® -4P
9
Chapter Overview
10
Disclaimer
11
Safety Summary
13
Risks of Ultraviolet Exposure
15
Additional Risks of Xenon Lamps
17
CE Compliance Statement
19
1 : Requirements & Installation
21
Safety-Training Requirements
21
Surface Requirements
22
Environmental Requirements
23
Electrical Requirements
24
Unpacking and Installation
25
Software Emulation
29
2 : System Description
31
Introduction
31
Basic Theory of Operation
31
Optical Layout
32
3 : System Operation
37
Introduction
37
Power Switch
37
Turning on the System
38
Checking System Performance
39
4 : Data Acquisition
53
Introduction to Fluoressence™ and Multigroup
53
Experiment Menu Button
54
Previous Experiment Setup Button
56
Auto Run Previous Experiment Button
57
Make Overlay File Button
58
3D Scan to 3D Profile Button
59
Run JY Batch Experiments Button
61
Real Time Control
63
Create/Use Calibration Curve from CWA Data
64
2D Intensity Map
68
Launch Datastation
70
Switch Menu between HJY Software Application and Origin Std
71
Multigroup
72
Running an Unknown Sample
75
Using Corrected Signals in Fluoressence
80
5 : Optimizing Data
83
Cuvette Preparation
83
Sample Preparation
84
Running a Scan on a Sample
86
Measuring the G Factor
87
Improving the Signal-To-Noise Ratio
89
Correcting Data
96
6 : Maintenance
103
Introduction
103
Lamp Replacement
103
Electronics
113
Updating the Fluoromax -4 Firmware
115
7 : Troubleshooting
119
Chart
119
Using Diagnostic Spectra
122
Further Assistance
127
8 : Producing Correction Factors
131
Introduction
131
Generating Emission Correction Factors
132
Calculating Emission Correction Factors
133
Using Correction-Factor Files
140
Excitation Correction Factors
143
9 : Fluoromax ® -4P Phosphorimeter Operation
145
Introduction
145
Theory of Operation
146
Applications for the Phosphorimeter
149
Operation of the Phosphorimeter
152
Processing Phosphorimeter Data
154
Lamp Replacement
155
10 : Automated Polarizers
161
Introduction
161
Installation
165
Alignment
166
Using Automated Polarizers
173
Maintenance
178
Troubleshooting
179
11 : Technical Specifications
181
Introduction
181
Spectrofluorometer System
182
Minimum Host-Computer Requirements
184
Software
184
12 : Components & Accessories
185
Itemized List of Fluoromax ® -4 Accessories
186
FL-1013 Liquid Nitrogen Dewar Assembly
187
Sample Cells
188
F-3026 Correction Factor Kit
190
F-3023 Janis Cryostat
191
1939 Cut-On Filter
193
FM-2008 Cut-On Filter Holder
194
FL4-1011 Four-Position Thermostatted Cell Holder
195
FL4-1012 Dual-Position Thermostatted Cell Holder
197
FL4-1027 Single-Position Thermostatted Cell Holder
199
J1933 Solid Sample Holder
201
F-3005/6 Autotitration Injector
203
1905-OFR 150-W Xenon Lamp
204
F-3004 Sample Heater/Cooler Peltier Thermocouple Drive
205
Micromax 384 Microwell Plate Reader
206
FM4-2000 L-Format Polarizer
207
FL4-1015 Injector Port
208
F-3029 Quanta-Φ Accessory
209
FM-2002 Shutter Accessory
210
F-3025 Stopped-Flow Accessory
211
FM4-2015 and FM4-2016 TCSPC Upgrade
212
F4-3030 Temperature Bath
213
F-3031 Transmission Accessory
214
J400981 Trigger Cable
215
FM-2007 Windows for the Fluoromax
216
13 : Glossary
217
14 : Bibliography
229
15 : Compliance Information
231
Declaration of Conformity
231
Supplementary Information
231
16 : Index
233
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Horiba Scientific FluoroMax-4 Specifications
General
Brand
Horiba Scientific
Model
FluoroMax-4
Category
Laboratory Equipment
Language
English
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