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INTELLICYT iQue Screener PLUS User Manual

INTELLICYT iQue Screener PLUS
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iQue
®
Screener PLUS
hardware manual
December 2016
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INTELLICYT iQue Screener PLUS Specifications

General IconGeneral
BrandINTELLICYT
ModeliQue Screener PLUS
CategoryLaboratory Equipment
LanguageEnglish

Summary

Introduction to the iQue Screener PLUS

Statement of Proper Use

Defines the intended purpose and usage environment for the iQue Screener PLUS system.

End User Use Agreement

Details the terms and conditions for using the iQue Screening System and associated products.

Software License Agreement

Outlines the terms and conditions governing the use of the iQue Screener System software.

Declaration of Conformity

States compliance with relevant European directives and product standards.

Using This Manual

Conventions

Explains terminology, conventions, and symbols used in the manual for clarity.

Terminology

Defines common terms used in the iQue Screener PLUS hardware manual.

Text Conventions

Details formatting conventions for menus, buttons, window titles, and dialog boxes.

Text Symbols

Explains symbols used to highlight important information, tips, and suggestions.

Safety Symbols

Illustrates safety symbols indicating hazards like DANGER, WARNING, and CAUTION.

Accessing Technical Support

Provides contact information and preparation steps for seeking technical assistance.

Safety Guidelines for iQue Screener PLUS

General Precautions

Highlights essential safety measures and warnings for product usage.

General Warnings

Outlines critical warnings regarding intended use, safety protection, and attire.

Operator Safety

Details safety precautions to prevent operator injury, including handling covers and moving parts.

Safety Symbols and Labels

Explains safety symbols and labels for understanding potential hazards and precautions.

Biological Handling Precautions

Provides guidelines for safely handling biological samples and reagents.

Laser Precautions

Details safety measures related to the instrument's laser system to prevent eye and skin exposure.

Operational Precautions

Covers precautions related to ambient conditions, area of use, and equipment setup.

Electrical Precautions

Highlights electrical safety measures, including proper power connections and UPS usage.

Getting to Know the iQue Screener PLUS

System Overview

Introduces the iQue Screener PLUS as a platform for screening cells, beads, microbes, and mixtures.

System Requirements

Lists the power, environment, and size requirements for the iQue Screener PLUS.

Instrument Variations

Describes the three available variations of the iQue Screener PLUS and their distinguishing features.

Technical Specifications

Details features like detection method, laser power, multicolor detection, and sample formats.

Fluorescence Channels - BR

Lists fluorescence channels, filters, and example dyes for the BR variant.

Fluorescence Channels - VBR

Lists fluorescence channels, filters, and example dyes for the VBR variant.

Fluorescence Channels - VYB

Lists fluorescence channels, filters, and example dyes for the VYB variant.

Front View Components

Identifies key components visible on the front of the instrument.

Status Light Indicator

Explains the meaning of the status light colors and their relation to system readiness.

Shaker Component Detail

Provides a detailed view and labels for the instrument's shaker mechanism.

Sampling Area Components

Details the components within the sampling area, including tube holders and fluid cartridges.

Sampler Mechanism Detail

Illustrates the probe assembly, sensors, and arms of the sampler.

Peristaltic Pump Detail

Shows the peristaltic pump housing, rollers, and tubing connections.

Right Side Panel Components

Identifies ports and connections on the right side panel of the instrument.

Bottle Station Configuration

Details the bottles for Sheath, PLUS ONE, PLUS TWO, and Waste fluids.

Fluidics Harness Detail

Shows the fluid station data cable and auto-closing quick connects.

Back Panel Components

Identifies power switch, fuse holder, power socket, and data port on the back panel.

Front Service Area Components

Highlights user-serviceable parts like the fluidlink connection and sheath filter.

Optical Bench - BR Variant

Illustrates the optical bench and components for the BR laser configuration.

PMT Module Detail - BR

Shows the Photomultiplier Tube (PMT) module details for the BR variant.

Optical Bench - VBR Variant

Illustrates the optical bench and components for the VBR laser configuration.

PMT Module Detail - VBR

Shows the Photomultiplier Tube (PMT) module details for the VBR variant.

Optical Bench - VYB Variant

Illustrates the optical bench and components for the VYB laser configuration.

PMT Module Detail - VYB

Shows the Photomultiplier Tube (PMT) module details for the VYB variant.

Operation Procedures for iQue Screener PLUS

Daily Startup Procedure

Step-by-step guide for performing the daily startup of the iQue Screener PLUS.

Daily Quality Control (QC)

Procedure for running daily quality control to ensure system performance and diagnose issues.

Interpreting Daily QC Results

Guidance on analyzing the results of the daily QC test, including dot plots and metrics.

Running a Sample Plate

Details the minimum steps required to run a simple plate, from preparation to analysis.

Prepare a Plate

Step 1: Guidance on preparing the sample plate for the experiment.

Create a New Experiment

Step 2: Instructions for creating a new experiment in the ForeCyt software.

Set the Acquisition Protocol

Step 3: Details on setting the prime, shake, and sip time for the acquisition protocol.

Run the Plate

Step 4: How to initiate and monitor the plate run on the instrument.

Perform Well Identification

Step 5: Process for identifying and gating wells for data analysis.

Analyze your Data

Step 6: Basic data analysis techniques using ForeCyt software and scatter plots.

Daily Shutdown Procedure

Procedure for safely shutting down the iQue Screener PLUS daily.

Bottle Fluids Management

Describes preparation and management of fluids in the bottle station.

Liquid Level Detection System

Explains how the bottle station monitors fluid levels and provides warnings or errors.

Deck Fluids Management

Details the fluids used in the sampling area, including cartridges and troughs.

Maintenance and Troubleshooting for iQue Screener PLUS

Recommended Maintenance Schedule

Outlines daily, weekly, monthly, and on-demand maintenance procedures.

Standard Cleaning Procedures

Explains various cleaning procedures accessible via the ForeCyt software Clean Wizard.

Probe and Tubing Clean

Cleans the probe and tubing using a sequence of Decon, Cleaner, and Water.

Detector Quick Clean

Clears deposits and bubbles from the flow cell and tubing after short inactivity.

Detector Long Clean

Thoroughly cleans the flow cell and tubing after heavy usage or long inactivity.

Detector Unclog Procedure

Procedure for suspected clogs in the detector and monthly preventative maintenance.

Detector Debubble Procedure

Clears air bubbles from detector fluidic lines and bottles.

Changing the Probe and Tubing

Describes the procedure for replacing the instrument's probe and tubing assembly.

Calibrating Deck Height

Recalibrates the deck height after a probe and tubing change.

Cleaning the New Tubing

Prompts to clean the probe and tubing after replacement and calibration.

Fluidics Maintenance

Details the replacement of filters and the FluidLink for optimal system performance.

Managing Plate Models

Discusses setting up and maintaining user-defined plate models for experiment protocols.

Setting Default Plate Model

Customizes which plate model is used by default when creating new experiments.

Deleting a Plate Model

Explains how to remove or deactivate plate models from the system.

Manual Control Mode

Allows direct interaction with instrument subcomponents for troubleshooting.

Testing Shaker RPM

Guidance on finding a safe shaker speed by testing various RPMs.

Verify Sample Position and Depth

Verifies proper sampling position and depth after mapping a plate model.

Troubleshooting Clogs

Isolates subsystems to diagnose and troubleshoot clogs in the probe or detector.

Clog Prevention and Management

Explains how to recognize, address, and prevent clogs in the system.

Special Topics for iQue Screener PLUS

Moving the iQue Screener PLUS

Provides procedures for safely shutting down and moving the instrument within a lab.

Connecting Power and Data Cables

Details the proper connection of power and data cables, including UPS usage.

Purge and Prime Procedures

Describes the process of purging fluids for transport and re-priming the system for use.