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General| Voltage Range | 20-25 V |
|---|---|
| Technology | Dry blotting technology |
| Application | Protein Transfer |
| Power Supply | 100-240 V AC |
| Transfer Time | 7 minutes |
| Compatible Membranes | PVDF, Nitrocellulose |
| Gel Size | Mini and midi gels |
Details about the components included with the iBlot™ 2 Gel Transfer Device.
Safety information and precautions to take before using the product.
Instructions and conditions for setting up the iBlot™ 2 Gel Transfer Device.
Procedures for safely unpacking the instrument upon receipt.
Overview of the front view of the iBlot™ 2 Gel Transfer Device.
General explanation of the system, its features, components, and operation.
Detailed descriptions of the individual components of the device.
Covers setting date/time, tutorials, logs, firmware, factory reset, methods, and parameters.
Technical specifications for the iBlot™ 2 Gel Transfer Device and stacks.
Information on compatible accessories and related products.
Outline of the steps for performing western blotting and general recommendations.
Lists compatible gel types for use with the iBlot™ 2 system.
Step-by-step instructions for assembling the device and performing blotting.
How to choose an appropriate blotting method on the device interface.
Procedure for carefully removing the gel from the cassette.
Detailed steps for correctly assembling the transfer stack components.
Instructions on how to conduct the actual protein transfer process.
Steps for taking apart the transfer stack after the blotting procedure.
Steps to create and program new blotting methods from scratch.
Procedure for naming and saving newly created custom methods.
Guide on using pre-set templates or saved methods as a starting point.
How to modify voltage and time settings for individual steps in a method.
Instructions for adding or deleting steps within a custom method.
Overview of common issues and how to resolve them.
Troubleshooting steps when the device does not detect current.
Solutions for problems like no protein transfer or empty spots on the membrane.
Addressing issues with distorted protein bands or protein blow-through.
Troubleshooting problems with protein binding, high background, and membrane discoloration.
Procedures after blotting, including immunodetection, storage, and staining.
Strategies for improving protein transfer efficiency through parameter adjustments.
Instructions for cleaning and routine upkeep of the instrument.
Procedure for replacing the device's fuse.
Step-by-step guide for replacing worn electrical contacts.
Detailed technical specifications for the main device.
Detailed technical specifications for the transfer stacks.
Ordering information for iBlot™ 2 Transfer Stacks.
Lists other available reagents, precast gels, and premade buffers.
General warnings and precautions for instrument operation, including physical and electrical hazards.
Safety precautions related to electrical hazards and power supply.
Safe procedures for cleaning and decontaminating the instrument.
Guidelines for safely handling chemicals and biological materials.
Explains instrument symbols and lists relevant safety and EMC standards.
Information on how to contact support for the product.
Details regarding the product's warranty policy.
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