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OPERATION: (Con’t)
TAKING A FYRITE READING: (Con’t)
Depress the plunger and hold it down. It is very important not to allow the
plunger to spring up—if this occurs, you will be repeating an already existing
reading. It will not matter how many times you pump the squeeze bulb, as long
as you hold the plunger down you will introduce only one sample to the Fyrite.
But, by releasing the plunger and immediately pushing it back down, you will
then be adding an additional sample to the Fyrite chamber causing the reading
to double or triple. In the event that it is difficult to hold the plunger down while
pumping the squeeze bulb, find a more suitable or comfortable position.
Without releasing the plunger, pump the squeeze bulb at least 20 times—
this assures complete and thorough transfer of the sample to the Fyrite chamber.
Once the bulb has been pumped the required number of times, HOLD THE
SQUEEZE BULB IN THE "SQUEEZED" POSITION and release the pressure on
the cup which will also release the plunger. This traps the sample from the
Incubator in the Fyrite chamber and allows the fluid to absorb the CO2.
With the plunger now released, turn the Fyrite upside down again and
hold at a 45-degree angle to allow the smaller bubbles to surface while the fluid
fills the chamber head. Reverse and turn Fyrite right side up and hold at a 45-
degree angle to allow the same thing to happen. Repeat this procedure—hold
upside down at 45-degree angle and right-side up at 45-degree angle. Finish by
shaking the Fyrite slightly while holding at 45-degree angle to allow residue
droplets of fluid to drain into the measuring tube.
Read the gauge: It should match or be close to your CO2 set point. It is
possible that you may be off as much as ±3%. If you read more than 8%, it is
possible that you may have introduced two samples or the Incubator is way out
of calibration. If a wide variation between the reading and CO2 set point exists, it
is recommended that the sampling and measurement procedure be repeated.
Once the possibility of any error in Fyrite reading has been eliminated,
calibrate your Incubator to conform with the reading obtained.
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