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Libra S22, English 3
The instrument calculates concentration, displays 260/280 and 260/230 ratios, and
compensates for dilution and use of cells that do not have 10mm pathlength. A
wavelength scan of a sample can also be obtained for visual inspection of integrity.
The procedure is as follows for DNA (3.1), RNA (3.2) and oligo (3.3):
• Enter pathlength of cell; 10mm (1), 5mm (2), 2mm (3), 1mm (4) or 0.5mm (5)
• Select units; µg/ml (1), ng/µl (2) or µg/µl (3)
• Select if background correction at 320 nm is required
• Select if sample scan is required (scans 220 to 330 nm, with autoscaling)
• Enter dilution factor
• [Oligo (3.3) only; enter conversion factor. If not known, use 33]
• Insert reference and press green run key
• This reference scan is used for subsequent samples until changed
• Insert samples as required and press (repeat as necessary)
• To go back and change the parameters press Method (F1)
• Press Graph to view the sample spectrum
Please note that the Libra S22 is not compatible with
standard 70µl UV disposable cuvettes
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