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Miltenyi Biotec autoMACS Pro User Manual

Miltenyi Biotec autoMACS Pro
166 pages
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autoMACS® Pro Separator
User manual
Version 5
Original instructions

Table of Contents

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Miltenyi Biotec autoMACS Pro Specifications

General IconGeneral
BrandMiltenyi Biotec
ModelautoMACS Pro
CategoryWater Filtration Systems
LanguageEnglish

Summary

Important Safety Information

Warnings, Precautions, and Hazard Levels

Explains potential risks, safety guidelines, and defines hazard level signal words.

Safety Symbols, Labels, and General Instructions

Details safety symbols, label locations, and general safety instructions for safe operation.

Specific Hazard Categories and Handling

Covers electrical, fire, magnetic, mechanical, optical, chemical, and biological hazards and handling.

Servicing, Transport, and Disposal Guidelines

Provides guidance on instrument maintenance, transportation, and proper waste disposal procedures.

1 Introduction

1.1 MACS® Technology: The Gold Standard

Explains MACS Technology basis: MicroBeads, Columns, and Separators for cell separation.

1.2 The autoMACS Pro Separator

Introduces the autoMACS Pro Separator as a benchtop cell sorter for automated sample processing.

2 Installation

2.1 Components and Materials for Operation

Lists kit components and necessary materials for daily operation and setup.

2.3 autoMACS Pro Separator Accessories

Lists optional accessories like Chill Racks, Hood Plates, and Connector Sets for enhanced functionality.

2.4 Unpacking and 2.5 Installing Fluid Bottles

Guides on safely unpacking the instrument and correctly installing fluid bottles.

2.6 Installing MACS MiniSampler and Racks

Details the correct procedure for installing the MACS MiniSampler, Reagent Rack, and tube racks.

3 Switching on or off

3.1 Switching on the Instrument

Covers initial power-on, first-time setup, and priming procedures for safe operation.

3.1.1 Initial Instrument Setup Steps

Details syringe installation, column setup, date/time setting, scanner test, and needle calibration.

3.2 Shutting Down the Instrument

Outlines procedures for safely powering down the instrument for sleep mode or long-term storage.

4 The autoMACS® Pro user interface

4.1 The Status Menu

Details how the status menu displays instrument and component status using color-coded symbols.

4.2 The Reagent Menu

Explains how to assign reagents using manual entry or a 2D code reader.

4.3 The Separation Menu

Describes setting sample rack templates, labeling, volume, separation, and wash programs.

4.4 The Log list menu

Provides access to a log of completed actions, errors, and detailed program status information.

4.5 The Option menu

Covers maintenance procedures (Special) and user settings for calibration and configuration.

5 Calibration

5.1 Calibrate the Needle Position

Guides on calibrating the needle arm's axes for accurate sample and reagent uptake.

5.1.1 Running a Test Calibration

Describes the step-by-step process for testing the needle's position in the washing station and tubes.

5.2 Calibrating the Uptake Volume

Explains the automatic calibration of the liquid volume control for accurate sample processing.

6 Cell separation

6.1 Cell Labeling Strategies

Explains direct and indirect magnetic labeling approaches using MACS MicroBeads.

6.2 Cell Separation Strategies

Details basic strategies: positive selection, depletion, untouched isolation, and sequential sorting.

6.3 Separation Programs

Lists and describes the fourteen pre-set separation programs available on the instrument.

6.4 Wash and Maintenance Programs

Lists wash programs (Qrinse, Rinse, Clean, Sleep) and maintenance programs (Safe, Store, Col_ex).

6.8 Monitoring the Separation Process

Explains how to monitor instrument status, program progress, and fluid bottle illumination.

6.10 Cell Labeling and Separation

Covers manual and automated cell labeling, program selection, and sample processing.

6.11 Shutting Down the Instrument

Details the process for shutting down the instrument, including sleep mode and long-term storage preparation.

7 Maintenance

7.1 Daily Maintenance Procedures

Outlines essential daily tasks like instrument priming and cleaning ports for optimal performance.

7.2 Periodic Maintenance Tasks

Covers regular tasks like column exchange, filter replacement, syringe cleaning, and disinfection.

7.3 Corrective Maintenance Procedures

Addresses maintenance for fixing issues like valve or pump head problems, tubing exchange, and fuse replacement.

8 Quality Control

8.1 Cell Recovery Calculation

Explains how to calculate target and overall cell recovery percentages after separation.

8.2 Purity of Isolated Cell Population

Details methods for analyzing and documenting the purity of isolated cell subsets.

8.3 Viability of the Cells

Describes how to assess cell viability using different dyes and methods.

9 Troubleshooting

9.1 Issues with Moving Hardware

Recommends running the Check_up program to diagnose and report on moving hardware component functionality.

9.2 Hardware Issues Not Indicated by Error Screen

Provides solutions for common hardware problems like leakages and blockages without error codes.

9.2.10 Power Supply Disruption During Separation

Outlines cell rescue procedures (A and B) for recovering samples after power loss.

9.3 Hardware Issues Indicated by Error Screen

Lists error codes, their causes, and possible remedies for instrument malfunctions.

10 Technical Data and Specifications

10.1 Technical Data and Specifications

Details instrument specifications, operating conditions, power requirements, and sound pressure level.

10.2 FCC Compliance Rules

States compliance with FCC rules for digital devices and measures to correct interference.

10.3 EC Declaration of Conformity

Provides manufacturer information and confirms compliance with EU directives and harmonized standards.

11 Technical Support

Contacting Miltenyi Biotec Technical Support

Provides contact information for Miltenyi Biotec headquarters and local representatives for support.

12 Limited Warranty

Warranty Terms and Conditions

Outlines the scope of Miltenyi Biotec's product warranty and exclusions for defects and misuse.