NEW BRUNSWICK SCIENTIFIC BioFlo 415 User Manual

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BioFlo 415

Benchtop SIP

Fermentor

MANUAL NO: M1360-0050

Revision D

December 15, 2008

NEW BRUNSWICK SCIENTIFIC

BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005

Telephone: 1-732-287-1200 • 1-800-631-5417

Fax: 732-287-4222 • bioinfo@nbsc.com • Web: www.nbsc.com

Default Chapter7
- Table of Contents7
1 Warnings & Cautions13
- Warnings13
- Cautions13
2 Inspection & Unpacking of Equipment15
- Inspection of Box(Es)15
- Packing List Verification15
- Basic Components15
- Box(E)15
3 Introduction & Overview16
- System16
- Vessels16
- Agitation System16
- Temperature Control17
- Aeration17
- Ph Control17
- Do Control18
- High Foam Control18
- Exhaust System18
- Sampling/Harvesting System18
- Recommended Accessories & Supplies19
- Supervisory Software19
4 Installation20
- Physical Location20
- Environment20
- Installing the Control Cabinet20
- Installing the Touchscreen20
- Utilities22
- Vessel Assembly25
- Electrical Connections33
- Main Power Switch33
- Optional Biocommand Plus34
- Inputs/Outputs for Ancillary Devices34
5 Specifications36
- Certifications37
6 Operating Controls39
- Touchscreen39
- Display Screens39
- Rs232/422 Computer Interface51
7 Probe Preparation & Calibration54
- Ph Probe Inspection54
- Ph Probe Calibration54
  - Ph Probe Installation56
  - Ph Probe Maintenance & Storage57
- Dissolved Oxygen (Do) Probe Preparation57
8 Automatic Sterilization61
- Essential: Backflow Prevention Kit61
- Bioflo 415 • M136062
- Sterilization63
- Valve Sequencing68
- Sterilization Phase Indication70
- Aborting Sterilization70
9 Preparing for Fermentation71
- Systems Check71
- Adding Media to the Vessel72
10 Getting Started73
- Control Modes73
- Pump Assignment73
- Settingp & I Values74
- Loop Setpoints74
  - Entering Setpoints74
  - Modifying Setpoints75
- Do Cascade System75
- Using Level Probes to Program Feed Pumps76
11 Cascade Control79
- Creating a Cascade79
- Controlling Do by Cascade81
12 Plotting Trends84
- Creating a Trend Graph84
13 About Pumps89
- Pump Setpoint89
- Pump Control Mode90
- Pump Flow Rate & Calibration Methods91
- Pump Period92
- Installing an External Variable Speed Pump92
14 About Alarms94
- Abs and Dev Alarms94
- Setting Alarms94
- Acknowledging an Alarm96
- Alarms History97
15 Using the Setup Screen99
- Controller Setup99
- Recipe105
- System Settings105
  - Resetting Date/Time106
  - Updating Software106
- Hardware Setup106
- Security Settings108
16 Operation110
- Load Pump Tubing110
- Install Liquid Addition Systems112
  - Addition Tubing Size113
- Inoculation114
- Launch Biocommand (if Present)114
- Sampling Procedure114
- Harvesting116
- Fermentation Phases116
- Batch Operation117
- Fed Batch Operation117
- Chemostat Operation118
- Shutdown Procedure119
17 Cleaning120
- Cleaning the Vessel120
  - List of Wetted Parts120
- Cleaning the Cabinet121
18 Maintenance122
- Ph Probe Maintenance and Storage122
- Do Probe Maintenance and Storage122
- Vessel & Tubing123
- Periodic Inspection123
- Fuse Replacement123
- Port & Adapter O-Rings123
- Headplate O-Ring Replacement123
- Replacement Parts124
- Part124
19 Service125
- Troubleshooting125
- Service Parts127
- Part127
20 Drawings128
- List of Drawings131
- List of Tables133
  - Table133
21 Appendix A: some General Concepts134
- What Is a Controller134
- What Is a Control Loop134
- What Is Probe Calibration135
- What Are P-I-D Constants135
- What Is P-I-D Tuning136
- What Do the Constants Mean137
22 Appendix B: Otr138
- Determining an Oxygen Transfer Rate138
  - OTR Calculations138
- Some Factors that Affect Otr and Horsepower139
23 Appendix C: Fermentation Techniques140
- Media Formulation140
- Antifoam Formulation141
- Tubing Size141
- Acid & Base142
- Glucose Feed142
- Recommended Process Control Settings143
24 Appendix D: Corrosion Resistance Tables144
25 Appendix E: General Characteristics of Epr159
- Identifying Epr159
- General Characteristics159
26 Index160

Questions and Answers:

M
Marcus MillsAug 18, 2025
Why are my DO probe readings erratic on my NEW BRUNSWICK SCIENTIFIC BioFlo 415 Test Equipment?
- S
  Steve BryantAug 18, 2025
  Erratic DO probe readings can be addressed by: * Recalibrating the probe, carefully following the instructions in the manual. * Recharging the probe, carefully following the instructions in the manual. * Replacing the probe membrane and refilling the electrolyte, if needed. * Checking for a secure connection. * Replacing the probe cable or the DO probe.