NEW BRUNSWICK SCIENTIFIC BioFlo 415

Questions and Answers

M
Marcus MillsAug 18, 2025
Why are my DO probe readings erratic on my NEW BRUNSWICK SCIENTIFIC BioFlo 415 Test Equipment?
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  Steve BryantAug 18, 2025
  Erratic DO probe readings can be addressed by: * Recalibrating the probe, carefully following the instructions in the manual. * Recharging the probe, carefully following the instructions in the manual. * Replacing the probe membrane and refilling the electrolyte, if needed. * Checking for a secure connection. * Replacing the probe cable or the DO probe.
W
Walter CraigAug 20, 2025
Why are my pH probe readings erratic on my NEW BRUNSWICK SCIENTIFIC BioFlo 415?
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  Veronica JohnsonAug 20, 2025
  Erratic pH probe readings can occur for several reasons. You can try: * Recalibrating the probe, carefully following the instructions in the manual. * Ensuring a secure connection. * If you have a gel-filled probe, it may need replacement. * If it’s a liquid-filled probe, it might need a refill of electrolyte. * The probe cable may also need replacement.
C
Chad HarveyAug 23, 2025
What to do if my NEW BRUNSWICK SCIENTIFIC BioFlo 415 unit will not heat up?
- O
  ortizsummerAug 23, 2025
  If your unit isn't heating up, consider the following: * Ensure the unit was properly primed at start-up. * Verify the temperature probe is correctly placed in the vessel thermowell. * Check that the water pressure is within the recommended range; increase if too low. * Confirm the correct connection (click to lock) of the water inlet and outlet lines on the vessel heat exchanger.
J
Jennifer FreemanAug 26, 2025
What to do if there is insufficient gas flow in my NEW BRUNSWICK SCIENTIFIC Test Equipment?
- G
  gomezmatthewAug 26, 2025
  If you're experiencing insufficient gas flow, you can try the following: * Replace the inlet or exhaust sterile air filter if it's wet or clogged. * Check that the air pressure is within the specified range. * Ensure the control mode for DO and pH is set to AUTO (not OFF). * Make sure the GasFlow loop is ON. * Verify that the Air loop is in O2 Enrichment mode. * Confirm that the DO cascades are Enabled.
M
Miss Stacey Thompson DDSAug 28, 2025
What to do if my NEW BRUNSWICK SCIENTIFIC BioFlo 415 Test Equipment touchscreen is not responding?
- S
  Stephanie GreenAug 28, 2025
  If the touchscreen is not responding, calibrate the touchscreen.
L
lclarkAug 30, 2025
What to do if my NEW BRUNSWICK SCIENTIFIC BioFlo 415 Test Equipment unit does not reach sterilization temperature?
- M
  Michelle EdwardsAug 30, 2025
  If the unit fails to reach sterilization temperature, it might be due to high water pressure (greater than 10 PSIG), causing the relief valve to open on the jacket. Try lowering the pressure and restarting the sterilization cycle.
K
Keith PhillipsSep 1, 2025
What to do if my NEW BRUNSWICK SCIENTIFIC BioFlo 415 probe does not hold calibration?
- S
  stevemontgomerySep 1, 2025
  If the probe isn't holding calibration, the probe may be defective and should be replaced.
B
bradley61Sep 4, 2025
What to do if the agitator does not turn or turns slowly on my NEW BRUNSWICK SCIENTIFIC BioFlo 415?
- K
  Kimberly SmithSep 4, 2025
  If the agitator isn't turning or is moving slowly, ensure the motor is plugged into the cabinet receptacle. Remember to turn off summary power before connecting the plug.
H
heather91Sep 6, 2025
What to do if my NEW BRUNSWICK SCIENTIFIC Test Equipment is leaking water?
- G
  Gabriel WatsonSep 6, 2025
  If the unit is leaking water, check for any loose connections on the inlet hoses and tighten them if necessary.
A
Andrew MillerSep 9, 2025
What to do if my NEW BRUNSWICK SCIENTIFIC BioFlo 415 readout is a negative value?
- K
  Kathryn LawrenceSep 9, 2025
  If the readout displays a negative value (typically –225° C), inspect the temperature probe for any visible damage and replace it if needed. Also, ensure the temperature probe is securely connected to the cabinet connector.

Summary

WARNINGS & CAUTIONS

WARNINGS

Lists critical safety warnings for operation and maintenance of the BioFlo 415.

CAUTIONS

Lists important precautions to prevent equipment damage or personal injury.

INSPECTION & UNPACKING OF EQUIPMENT

Inspection and Verification

INSTALLATION

Location and Environment

Cabinet and Touchscreen Setup

Instructions for installing the control cabinet and touchscreen monitor.

Utilities and Connections

Covers connections for gas, water, electrical, and ancillary devices.

Vessel Assembly

Details on assembling the vessel and connecting it to the cabinet.

Electrical and Power Connections

Instructions for connecting the vessel heater, power cord, and main switch.

SPECIFICATIONS

System Specifications

OPERATING CONTROLS

Touchscreen Interface

Navigation and System Screens

Covers key display screens like Summary, Synoptic, Gauge, Calibration, Cascade, Trend, Pump, Alarm, and Setup.

RS232;422 COMPUTER INTERFACE

PROBE PREPARATION & CALIBRATION

pH Probe Procedures

Covers pH probe inspection, calibration, installation, and maintenance.

DO Probe Procedures

Covers DO probe inspection, preparation, installation, polarization, and calibration.

AUTOMATIC STERILIZATION

Essential Safety Requirements

Importance and installation of the backflow prevention kit.

Sterilization Process Steps

Checklist and procedures for initiating, starting, and aborting sterilization.

Sterilization Configuration and Parameters

How to configure parameters, valve sequencing, and phase indication.

GETTING STARTED

Control Modes and Pump Operations

Explains control modes, pump assignment, P&I values, and setpoints.

Using Level Probes and Exhaust Heater

How to program feed pumps with level probes and activate the exhaust heater.

CASCADE CONTROL

Creating and Controlling Cascades

Step-by-step guide to setting up and controlling cascades, including DO examples.

PLOTTING TRENDS

Creating and Analyzing Trend Graphs

ABOUT PUMPS

Pump Setup and Calibration

External Variable Speed Pump Installation

ABOUT ALARMS

Alarm Types and Configuration

Alarm Management

USING THE SETUP SCREEN

Controller and Recipe Management

System and Hardware Configuration

Security Settings

OPERATION

Pre-Run Preparations

During and Post-Run Procedures

CLEANING

Vessel and Cabinet Cleaning

MAINTENANCE

Probe Maintenance and Storage

Component Checks and Replacements

SERVICE

Troubleshooting Guide

A guide to diagnosing and resolving common operational problems.

APPENDIX A: GENERAL CONCEPTS

P-I-D Control Theory

APPENDIX B: OTR

Oxygen Transfer Rate Determination

Factors Affecting OTR and Horsepower

APPENDIX C: FERMENTATION TECHNIQUES

Media and Additive Formulations

Recommended Process Settings