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CASY TT Operator‘s Guide
Points to be Observed when Performing Measurements
Depending on the selected setting (see Chapter .), the following para meters may also
be displayed:
Counts/ml: Particle concentration in the evaluation cursor size range.
%Counts: Percentage of particles in the evaluation cursor size range relative to the normalization
cursor size range (100%).
TotCnt/ml: Particle concentration in the normalization cursor size range.
%Volume: Percentage of volume in the evaluation cursor size range relative to the normalization
cursor size range (100%).
TotVol/ml: Volume in the normalization cursor size range.
The calculation of the peak diameter PeakDia and the peak volume PeakVol is based on a
sliding weighted mean value. The algorithm will perfectly match each peak width through a ve-
stage iteration and guarantees an accurate calculation of the maximum. The peak volume deter-
mined in this way is also used to automatically calculate the aggregation factor.
. Points to be Observed when Performing
Measurements
For the measuring operation, use only CASY ton, CASY cups and CASY clean specied for the
CASY System by OLS OMNI Life Science GmbH & Co. KG. Using alternative measuring solutions,
cups and cleaning agents may lead to incorrect measuring results and cause damage to the uid
system of the CASY Instrument. OLS OMNI Life Science GmbH & Co. KG accepts no liability for
damage caused by using alternative consumables.
A smooth measuring operation depends on the system undergoing the regular Weekly Cleaning
Routine (see Chapter 4.1) using CASY clean (see Chapter 4.1)!
You must ensure that your CASY System is also kept clean between cleaning cycles. Specically that
cells never remain in the uid system for long periods. The daily background monitoring procedure
(see Chapter 3.4) and the visual check of the vertical tube will indicate when the system is becoming
contaminated and requires cleaning.
The vertical tube is a calibrated glass pipette through which the CASY System, controlled by a light
barrier, aspirates the set measuring volume. The vertical tube is visible in the inspection window at
the front of the measuring unit. During the measurement, you can visually track the rising level of
the uid meniscus. Depending on the level in the vertical tube, one or several light diodes (Green
= start of measurement, Red = 200 µl measuring volume, Red = 400 µl measuring volume)
illuminates. Judge the status of the vertical tube as you would a glass pipette. If you see that the
uid is no longer running cleanly and drops of uid remain suspended on the edge, the calculation
of your sample concentration may be adversely aected. In such cases, run a cleaning cycle using
CASY clean (see Chapter 4.1).
Changing the samples
When you are measuring samples of the same cell type in a similar concentration range, it is not
necessary to ush the system when the samples are changed. If you expect larger concentration dif-
ferences or wish to perform measurements with a dierent sample type before a new measurement,
place a CASY cup containing freshly ltered CASY ton below the measuring capillary and ush the
system using the CLEAN function.
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