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ProteinSimple Maurice CE-SDS User Manual

ProteinSimple Maurice CE-SDS
13 pages
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Maurice® CE-SDS Application Guide
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ProteinSimple Maurice CE-SDS Specifications

General IconGeneral
BrandProteinSimple
ModelMaurice CE-SDS
CategoryLaboratory Equipment
LanguageEnglish

Summary

Introduction to Maurice CE-SDS

Storage Conditions for Reagents

Guidelines for storing reagents and standards at appropriate temperatures to maintain stability.

Ordering Information

Contact details for reordering the CE-SDS Size Application Kit and related products.

Other Necessary Items

List of additional consumables and equipment required for CE-SDS analysis.

Guidelines for Great Results

Cautionary Handling Advice

Handle the cartridge tip with extreme care to prevent damage and failed injections.

Application Overview

Details the benefits of a defined and optimized CE-SDS method, including peak profile and resolution.

Method Development Strategy

Step 1: System Performance Check

Maurice Setup and Start

Procedures for setting up the Maurice instrument and initiating a system performance check.

Expected Results Analysis

Reduced Sample Results

Details on expected peak profiles and migration times for reduced IgG samples.

Non-Reduced Sample Results

Details on expected peak profiles and migration times for non-reduced IgG samples.

Step 2: Set Up and Run Sample Batch

General Considerations for Sample Prep

Key factors for sample preparation, concentration, and composition for CE-SDS analysis.

Sample Preparation Methods

Protocols for preparing samples under reducing and non-reducing conditions.

Maurice Instrument Setup and Start

Procedures for reagent preparation and instrument setup for sample batch runs.

Optional MW Markers Preparation

Guidance for preparing CE-SDS Molecular Weight markers for optional use.

Step 3: Evaluate Your Results

Injection Profile Evaluation

Guidelines for assessing peak profiles, migration times, resolution, and impurity detection.

Method Optimization and Troubleshooting

Non-Reproducible Peak Profiles

Causes and solutions for non-reproducible peak profiles in CE-SDS analysis.

Noisy Baseline Resolution

Factors contributing to a noisy baseline and methods to resolve them.

Resolution and Signal Intensity Optimization

Techniques for optimizing peak resolution and signal intensity by adjusting injection conditions.

Resolution Troubleshooting

Troubleshooting common issues causing low peak resolution in CE-SDS analysis.

Separation Time and Throughput Optimization

Appendix A: Sample and Standard Preparation

Internal Standard Preparation

Detailed steps for preparing the 25X Internal Standard solution.

Reagent Preparation for Analysis

Protocols for preparing alkylation reagent and IgG standards for analysis.

Optional MW Markers and Sample Preparation

Procedures for optional MW marker preparation and final sample dilution.

Appendix B: Reagent Preparation

Batch Reagent Setup

Instructions for preparing and placing batch reagents in the Maurice instrument.

Appendix C: Desalting and Concentrating Samples

Appendix D: Cartridge Preparation

Top Running Buffer Vial Assembly

Steps for assembling the Top Running Buffer vial into the cartridge insert.

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