Verify that Sofia or
Sofia 2 is set to the
desired Mode:
WALK AWAY or
READ NOW. See
the “Using Sofia” or
“Using Sofia 2”
section for more
information.
Dispense all of the Reagent
Solution into the Reagent
Tube. Swirl the Reagent Tube
to dissolve its contents.
Fill the provided Large, Pink
250 µL Fixed Volume Pipette
with patient sample from the
collection cup.
To fill the Fixed Volume
Pipette with the sample:
a) FIRMLY squeeze the top
bulb.
b) Still squeezing, place the
Pipette tip into the patient
sample.
c) With the Pipette tip still in
the patient sample, release
pressure on bulb to fill the
Pipette.
NOTE: To obtain accurate
results, avoid mucoid
substances when filling the
Large, Pink 250 µL Fixed
Volume Pipette with patient
sample from the collection cup.
Firmly squeeze the top bulb to
empty the contents of the
Large, Pink 250 µL Fixed
Volume Pipette into the
Reagent Tube. Extra liquid in
the overflow bulb is OK.
NOTE: Once the sample is
added to the Reagent Tube,
vigorously mix prior to
adding the sample to the Test
Cassette.
NOTE: The Fixed Volume
Pipette is designed to collect
and dispense the correct
amount of patient sample.
Discard the Pipette in your
biohazard waste.
Fill the provided Small,
Clear 120 µL Fixed
Volume Pipette with
patient sample from the
Reagent Tube.
Firmly squeeze the top bulb
to empty the contents of
the Small, Clear 120 µL
Fixed Volume Pipette into
the Test Cassette sample
well. Extra liquid in the
overflow bulb is OK.
NOTE: Do not pour sample
from the Reagent Tube.
Use the provided Small,
Clear 120 µL Fixed Volume
Pipette.
Proceed to the
“Using Sofia” or
“Using Sofia 2”
section to complete
the test.
Liquid Specimens Test Procedure
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