Table of Contents
3
Prologue 5
I Revision History ............................................................................................................................................... 5
II Contact Addresses ........................................................................................................................................ 5
III Trademarks ........................................................................................................................................................ 6
IV Intended Use ..................................................................................................................................................... 6
V Conventions Used in this Guide ............................................................................................................. 6
VI Warnings and Precautions ........................................................................................................................ 8
A Start-up 11
1 Starting the MagNA Pure 96 Instrument ....................................................................................... 11
2 Starting the MagNA Pure 96 Software ........................................................................................... 12
3 Preparing the MagNA Pure System .................................................................................................. 13
3.1 System fluid ....................................................................................................................................................... 16
3.2 Waste containers ............................................................................................................................................. 20
B Main Applications 27
1 Setting up a Run for Purification of Genomic DNA from Whole Blood Samples .... 29
1.1 Preparing the Samples .................................................................................................................................. 29
1.2 Defining the Parameters in the Software ............................................................................................... 32
1.3 Loading the Stage ........................................................................................................................................... 34
2 Setting up a Run for Purification of Cellular RNA from Cultured Cells ......................... 43
2.1 Preparing the Samples .................................................................................................................................. 43
2.2 Defining the Parameters in the Software ............................................................................................... 44
2.3 Loading the Stage ........................................................................................................................................... 46
3 Performing a Run ......................................................................................................................................... 51
3.1 Starting a Run ................................................................................................................................................... 51
3.2 Monitoring the Run ........................................................................................................................................ 51
3.3 End of a Run ..................................................................................................................................................... 52
4 Unloading the Instrument ....................................................................................................................... 54
4.1 Handling Partially Used Reagent Sets ..................................................................................................... 56
4.2 Handling the Waste ........................................................................................................................................ 58
4.3 Cleaning .............................................................................................................................................................. 58
5 Performing a Volume Transfer ............................................................................................................. 59
5.1 Defining the Parameters in the Software ............................................................................................... 59
5.2 Loading the Stage ........................................................................................................................................... 61
C Shutting Down the System 67
D Appendix 69
1 Index ................................................................................................................................................................... 69
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