MagNA Pure 96 System, Customer Training Guide V1.0
44
Setting up a Run for Purification of Cellular RNA from Cultured Cells
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Defining the Parameters in the Software
To prepare suspension cells
Visually check the source plate to ensure that the samples are pipetted into each individual well of the
source plate.
To prepare the DNase solution
2.2 Defining the Parameters in the Software
Before starting a purification run, you must provide the software with the necessary information on pro-
tocol and sample parameters. The most important and obligatory information is:
Order type
Kit name
Purification protocol
Sample and elution volume
Number and name of samples
Once it has this information, the software calculates the type and amount of isolation reagents and dis-
posables.
Take the cell culture flask out of the cell culture incubator.
Shake the bottle slightly to resuspend the cells completely.
Transfer the cell suspension into a suitable centrifugation tube.
Centrifuge the cells for 10 minutes at 300 x g.
Discard the supernatant and resuspend the cell pellet in 200 µl PBS.
Pipette 200 µl cell suspension into the individual wells of a processing cartridge. This cartridge is then
defined as the source plate.
Pipette the samples into the bottom of the wells using aerosol-preventive tips.
Reconstitute two glass bottles of DNase (bottle 2), each with 3 ml from one bottle of DNase incubation
buffer (bottle 3).
Close both bottles and mix well by inverting them. After complete dissolution of the lyophilisate, a clear
to slightly opaque solution is obtained.
Do not vortex.
Transfer all the liquid back into the original plastic bottle labeled DNase incubation buffer (bottle 3).
Close the bottle with the original lid.
Check the box "DNase added" on the label of bottle 3 with a check mark.
Mix by inverting the bottles five times.
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