Quick Reference Guide
Tissue-Tek® Glas™ g2
QRG | Glas g2 | 2010 | V-5 page 7 | 11
Monthly
Pump anti-drying bottle
1. Change the solvent for mounting medium in
the pump anti-drying bottle.
Yearly
1. Preventive maintenance by a Sakura
representative or a qualified service engineer.
Table of coverslipped conditions and operator actions
To achieve a desired cover slipped condition, please see below:
Check the coverslipped slides to see if they are coverslipped in a satisfactory condition. If not, take
remedial actions by referring to the table below. For remedial actions to be taken in other situations, such
as when two or more coverglasses are picked up simultaneously or when coverglasses crack when they
are picked up, please refer to Section 6 in Troubleshooting.
Large air bubbles are
present on slides.
Not enough mounting medium is
dispensed.
Increase the dispense amount of mounting
medium.
Air bubbles in the dispensing channel of
mounting medium; mounting medium is
not dispensed by the specified amount.
Clear mounting medium channel by using the
PRIME key or use initial priming operation to
remove air bubbles.
Mounting medium has run out.
Replace the mounting medium bottle with a
full one. If the mounting-medium level
detection function is not working, the system
must be adjusted. Call the Customer Service
Center (CSC) or Sakura dealer.
The coverglass size does not match the
applicable settings on the system side.
Change the coverglass size displayed on the
screen according to the size of the coverglass
used.
There is not enough or no clearing agent in
the loading station.
Fill the loading station with clearing agent up
to the specified level.
The tissue thickness does not match the
applicable settings on the system side.
Increase the amount of mounting medium.
Alternatively, decrease the coverslipping
speed and examine appropriate coverslipping
conditions again. If the tissues do not have
an even thickness, air bubbles cannot be fully
eliminated.
The coverslipping applicator pressure is too
weak and needs to be increased.
Increase the applicator pressure of the cover
glass during coverslipping. For details,
consult the CSC or Sakura dealer.
Small air bubbles are
present.
Mounting medium did not spread effectively
because the ambient temperature was low
which increases viscosity allowing air
bubbles to enter as a result.
Decrease the coverslipping speed.
The tissue thickness does not match the
applicable settings on the system side.
Increase the amount of mounting medium.
Alternatively, decrease the coverslipping
speed and examine appropriate coverslipping
conditions again.
Dispensing nozzle cleaning
Cleaning the loading station
Cleaning the receiving racks
Checklist maintenance (short summary)
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