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General| Technology | Extracellular Flux Analysis |
|---|---|
| Measures | Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) |
| Injection Ports | 4 |
| Sample Throughput | 8 samples per run |
| Application | Cell metabolism studies, mitochondrial function, glycolysis |
| Sample Format | Adherent cells or suspension cells in a microplate |
| Sample Volume | 180 µL per well (recommended) |
| Well Plate Format | 8-well miniplate |
| Software Compatibility | Wave software |
Verifies all included items are present and checks for shipping damage.
Guidelines for placing the analyzer in a suitable laboratory environment.
Critical warnings and precautions for safe operation of the instrument.
Procedure for powering the instrument on and allowing it to reach operational temperature.
Steps for the first-time power-up and initial diagnostic tests.
Instructions for connecting the analyzer to a network for file transfer and support.
Correct procedure for shutting down the instrument completely.
Steps to configure and start an assay on the analyzer.
Instructions for confirming assay groups and plate layout settings.
Guide to checking and modifying assay protocol steps and cycles.
Process for reviewing assay details and initiating the analysis.
Procedure for loading components and starting the assay calibration.
Explains how to view and interpret acquired OCR and ECAR data.
Steps for finishing the assay and ensuring data is saved correctly.
Configure temperature tolerance, alarms, and atmospheric pressure.
Save copies of assay performance within the last 60 days to storage.
Define the default naming convention for assay result files.
Transfer assay template files between the analyzer and a Windows computer.
Guides configuration of network and other device settings.
Perform system checks, measurement system tests, thermal performance, and advanced measurements.
Verify barcode reading, calibration, and injection system performance.
Enable remote access for technical support.
Covers probe cleaning, tray and probe movement, and cartridge eject/load.
Provides worldwide contact details for Seahorse Support.
Displays installed software and firmware versions; checks for updates.
How to access help text related to the current screen.
Details integrated Ethernet adapter and approved Wi-Fi adapters.
Guides connection via Ethernet cable or wireless USB adapter.
Steps to configure wired network connection using IP address assignment.
Steps to connect the analyzer to a wireless network using a USB adapter.
Configure network directory for saving templates and results.
Configure email notifications and synchronize date/time settings.
Troubleshoot slow appearance of the template page due to network issues.
Resolve "load error" messages related to cartridge status.
Contact information for product inquiries and technical support.
Details on how to order XFp FluxPaks and other supplies.
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