Shimadzu UV-VIS User Manual

Shimadzu UV-VIS User’s Guide
1) Push the F4 button on the UV-VIS instrument keypad. This will enable PC
control.
» Push the F4 Button
2) Log into the UV-VIS software with your username and password.
3) After the UV-Probe software comes up, click on the “Connect” button near the
bottom of the screen to connect with the spectrometer.

Shimadzu UV-VIS Specifications

General

Device Type	UV-Vis Spectrophotometer
Detector Type	Silicon photodiode
Wavelength Range	190-1100 nm
Photometric Repeatability	±0.001 Abs
Light Source	WI lamp and D2 lamp
Display	LCD
Data Output	USB
Power Requirements	100-240 VAC, 50/60 Hz
Spectral Bandwidth	1 nm

Summary

How to Work in Spectrum Mode

Set Acquisition Parameters

Configure spectrum acquisition parameters like wavelength range and scan speed.

Zero Baseline

Zero the baseline with no sample or reference, ensuring the lid is closed.

Insert Sample and Reference

Place sample and optional blank cuvette in the sample and reference compartments.

Start Spectrum Collection

Click "Start" to begin collecting spectral data and save the file.

How to Label Peaks on Spectrum

Activate Peak Pick Tool

Click the "Peak Pick" button to display the Peak Pick table.

Configure Peak Properties

Adjust peak labeling parameters by right-clicking the Peak Pick table and selecting "Properties".

Print Peak Information

Preview and print peak information using "File, Print Preview".

Convert Spectra to ASCII Text Format

Access Data Print

Click the "Data Print" button to view raw data points in a table.

Set Data Print Properties

Ensure "All Data" is selected in "Data Print Properties" via right-click.

Save as ASCII File

Save data using "File, Save As", selecting "Data Print Table (*.txt)" file type.

How to Shut Down the Instrument

Save Data and Disconnect

Ensure data is saved, then click the "Disconnect" button.

Return to Local Control

Press the "Mode" button to return local control and turn off the deuterium lamp.

How to Work in Photometric Mode

Select Photometric Mode

Click the photometric button and "M" button to change method parameters.

Set Wavelengths

Enter desired wavelengths in the Photometric Method Wizard.

Configure Calibration Parameters

Set concentration units, calibration wavelength, and curve fit order.

Set Standard Measurement Parameters

Ensure "Data Acquired By: Instrument" is selected for standards.

Set Sample Measurement Parameters

Ensure "Data Acquired By: Instrument" is selected for samples.

Fill Standard and Sample Tables

Enter concentration information for standards and names for samples.

Baseline and Cell Blank Correction

Perform baseline correction and cell blanking before measurements.