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Soft Imaging System ColorView III User Manual

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analySIS
Manual
ColorView III
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Soft Imaging System ColorView III Specifications

General IconGeneral
BrandSoft Imaging System
ModelColorView III
CategoryDigital Camera
LanguageEnglish

Summary

The ColorView III - camera

Definition

Defines the ColorView III camera as a high-resolution CCD color camera for biomedical and material sciences.

Handbook Layout

Camera and light microscope

White Balance

Explains how color channel settings and white balance are crucial for acquiring quality images without color tinting.

White Balance

Executing White Balance

Provides instructions for executing white balance using transmitted or reflected light methods.

Online shading correction

Online shading correction

Explains how optical systems create image inhomogeneity (shading) and its correction.

Operation of the Shading Correction

Shading Correction

Details how image errors are determined and corrected in real-time using online shading correction.

Operation of the Shading Correction

Explains the principle of online shading correction and reference image acquisition.

Acquiring Correction Images

Acquiring Correction Images

Guides on acquiring correction images for online shading correction using a step-by-step wizard.

Transmitted Light Mode

Details the procedure for acquiring correction images in transmitted light mode.

Acquiring Correction Images

Reflected Light Mode

Guides on acquiring correction images in reflected light mode, including sample choice.

Enabling Shading Correction

Enabling Shading Correction

Explains how to activate or deactivate online shading correction after reference acquisitions.

Use of the Online Shading Correction

Use of the Online Shading Correction

Provides instructions for optimal acquisitions using online shading correction.

Image Acquisition

Image Acquisition

Covers various acquisition methods available with the image-analysis software.

Acquisition Methods

Lists and describes the different acquisition methods offered by the software.

intelligent Exposure

Details automated image acquisition coordinating signal amplification and exposure time.

Camera Control

Sharpness Monitor

Allows control of focus settings during live acquisition and defines an area for sharpness analysis.

How to...

Step-by-step guide on setting the sharpness monitor for good acquisitions.

Partial Readout

Method for fast live acquisition by defining a rectangular part of the image for readout.

Camera Control

Command to alter settings for acquisition with the camera.

Exposure Time

Explains setting exposure time for high-quality acquisitions based on illumination and sample.

Camera Control

Automatic Gain Display and Scaling

Explains how ColorView III uses dynamic range for display and editing, and methods for adjustment.

Color Settings

Explains how to manually alter camera's color settings using slide controls.

Gamma

Explains using Gamma slide control for nonlinear contrast enhancement.

Camera Control

Black balance

Explains black balance for background correction by subtracting mean values from pixels.

Snapshot

Snapshot

Acquires a single image and makes it available for image-analysis software.

intelligent Exposure

Procedure to make acquisitions with little effort, automating exposure times.

intelligent Exposure

Calibration

Explains intelligent Exposure's calibration process for measuring dark current and sensitivity.

intelligent Exposure

Exposure adjustment

Slide control to manually influence exposure time for snapshots.

NetCam

NetCam

Enables transferring ColorView III live-image via intranet or internet to other computers.

Installing the web server

Installing the web server

Describes automatic installation of the web server required for NetCam.

NetCam in the intranet

Discusses image transfer within a company network or intranet.

NetCam in the internet

Determining the IP address

Guides on how to determine your computer's IP address using Command Prompt.

Using NetCam

Using NetCam

Explains that image-analysis software functions remain the same when using NetCam.

NetCam Settings

Button to set image options and administer clients with access to images.

Using NetCam

Image options

Set the quality of transferred images and influence the transfer rate.

Usage in Black & White mode

Usage in Black & White mode

Describes changes when using a color camera in black & white mode.

Gamma Correction

Explains using gamma correction for physiological correction of camera non-linearities.

Display and usage of the acquisition with Real time FFT

Guides on utilizing Real time FFT during live acquisition.

Display and usage of the acquisition with Real time FFT

Enabling Real time FFT

Explains how to activate Real time FFT in the input channel.

Camera Set Up

Camera Set Up

Describes the automatic creation of an input channel upon software installation.

Software Camera Control

Explains how to configure the camera for image-analysis software after installation.

Setting up an input channel for black & white use

Details setting up a new input channel, specifically for black & white mode.

Software Camera Control

The Input tab

Details the settings available in the Input tab of the Configure Input dialog box.

Live overlay

Check box to use overlays in live-images, necessary for RFTFT and partial readout.

Exposure

Setting the number of microseconds the CCD chip is exposed.

Image scaling

Selects one of four options for displaying an acquired image in a Viewport.

Online shading correction

Setting the camera function for online shading correction.

Histogram calculation

Selects pixels for gray-value histogram calculation (Full image, Crosshairs, ROI).

Software Camera Control

Sharpen filter

Activating the sharpen filter to enhance image sharpness in real time.

Clipping border (pixel)

Explains clipping edges of images, affecting resolution and brightness.

Software Camera Control

Automatic gain display

Functions for optimal on-screen image display regardless of lighting conditions.

Online histogram

Check box to monitor gray-value distribution during image acquisition.

The Format tab

Details settings in the Format tab of the Configure Input dialog box.

Real time FFT

Check box available in B&W mode for using Real time FFT during live acquisition.

Fixed scaling

Manually influencing on-screen image display by selecting definite limits for gray-value range.

The Display tab

Details settings in the Display tab of the Configure Input dialog box.

Over exposure

Group to determine when and if to be warned of overexposure.

Appendix I: Microscope Settings

Appendix I: Microscope Settings

Guides on setting up the microscope for high image quality with digital cameras.

Illumination

Explains light's role in creating images and the importance of illumination settings.

Illumination

Köhler Illumination

Prerequisite for successful light microscopy analysis enabling optimal, homogenous illumination.

Objective, Total Magnification and Useful Magnification

Objective, Total Magnification and Useful Magnification

Discusses selecting objectives and key parameters like magnification and numerical aperture.

Appendix II: Camera Installation

Appendix II: Camera Installation

Guides on installing the ColorView III camera under Windows 2000 and XP.

General Warnings!

Lists general warnings about handling CCD sensors, static discharge, light exposure, etc.

The FireWire board IEEE-1394

Warns about static discharge damaging components and proper handling.

Installation Procedure - Windows 2000

Installation of the camera and cables

Reminds to install software before connecting camera to FireWire interface.

Installation Procedure - Windows 2000

Installation of the image-analysis software

Details installing the image-analysis software before connecting the camera.

Installation Procedure - Windows 2000

Precalibration

Advises carrying out camera precalibration during installation.

Installation of the camera and cables

Troubleshooting

Troubleshooting

Addresses problems when the camera cannot be found or is not detected.

What do I do if the camera cannot be found?

Explains error message when camera connection fails and checks for loose cables.

What to do when the camera has been connected before the image-analysis software installation

Addresses issues when Windows installs its own driver, preventing correct communication.

Installation Procedure - Windows XP

Installation of the FireWire board IEEE-1394

Explains when installation of the FireWire board is necessary for Windows XP.

How to install the FireWire board

Provides step-by-step instructions for installing the FireWire board.

Installation of the image-analysis software

Requirements, PC

Lists software and OS requirements for ColorView III camera under Windows XP.

How to install the camera

Provides diagram and steps for installing the camera assembly onto the microscope.

camera driver installation

Explains selecting FireWire and camera driver during setup for Windows XP.

Installation of the image-analysis software

Guides selecting FireWire digitizer and camera type during setup for Windows XP.

Installation of the camera driver

Installation of the camera driver

Guides on installing the SIS View FireWire camera driver under Windows XP.

When encountering problems with the camera...

When encountering problems with the camera...

Provides advice for contacting customer service for unresolved questions or problems.

CE DECLARATION OF CONFORMITY