Thermo Hybaid PX2

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THERMO HYBAID

Px2 Thermal Cycler

USER INSTRUCTION MANUAL

Head Office

Thermo Hybaid

Action Court

Ashford Road

Ashford

Middlesex TW15 1XB

Telephone: +44 (0) 1784 425 000

Facsimile: +44 (0) 1784 248 085

Internet: www.thermohybaid.com

STOCK CODE:

HB-PX2-MAN

FS31999

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Main Page

Pcr License Registration4
Warranty6
- Table of Contents8
Chapter 1: Introduction12
Introduction12
Overview12
Px2 System12
- The 0.2Ml Standard and Gradient Block Module12
- The 0.5Ml Standard and Gradient Block Module12
- The Flat Block Module12
- Heating and Cooling13
- Temperature Control Methods13
- The 384 Well Block Module13
- Advanced Edit - Temperature Ramping14
- Advanced Edit - Time/Temperature Increment14
- Pre-Set Programs14
- Programming and Operation14
- Advanced Edit - Gradient (Optional)15
- Calibration of Your Px215
The Heated Lid15
- Operating the Lid15
Input / Output Sockets16
Support Services17
Chapter 2: Safety Precautions18
Safety Precautions18
General Safety Precautions18
Symbols and Conventions19
Chapter 3: Unpacking & Installation20
Unpacking & Installation20
Unpacking20
Packing List20
Installation21
- Choosing a Location21
- Block Installation and Removal21
- Thermistor Positioning22
- Use in Cold Rooms22
Chapter 4: Operating Instructions24
Operating Instructions24
Initial Start up24
Main Menu24
Operating Keys25
Set up Functions25
- Clock25
- Power Failure Resume26
Gradient Calculation26
Running a Program27
Manual Operation27
Programming Function28
Loading Samples and Heated Lid Operation29
CHAPTER 5 PROGRAMMING the Px230
Introduction30
Directories30
- Creating a New Directory30
- Editing a Directory Name31
- Erasing an Existing Directory31
Programs32
- Creating a New Program32
- Editing an Existing Program33
- Viewing an Existing Program35
- Copying Programs35
- Erasing an Existing Program36
Advanced Edit Features36
- Time Advanced Worked Example37
- Temperature Advanced Worked Example38
- Changing Ramp Rates39
Gradient Feature39
- Gradient Programming Worked Example40
- Invalid Gradient Parameters40
- Gradient Calculator Function40
CHAPTER 6 RUNNING a PROGRAM on the Px243
The Run Option43
Running a Program on the Px2 - Short Instructions43
Run Screens44
- Text Screen44
- Power Failure Screen45
- End of Run Screen45
- Error Screen45
Temperature Control Options45
- Active Tube Control (ACTIVE TUBE)45
- Extension Lead for Tube Thermistor46
- Simulated Tube Control (SIM TUBE)47
  - Simulated Plate Control (SIM PLATE)47
- Simulated Slide Control (SIM SLIDE)48
- Block Control49
Heated Lid Preheat49
- Heated Lid - Automatic Start49
- Heated Lid - Manual Start49
- No Heated Lid50
Hold and Pause Function50
Aborting Programs50
Program Completion51
Chapter 7: Troubleshooting53
Troubleshooting53
Programs Using ACTIVE Tube Control53
- Check the Volume in the Tube Thermistor53
- Check the Location of Thermistor Within the Control Tube53
- Check the Fit of the Tube in the Block53
- Check through Program53
Programs Using SIM Temperature Control54
- Simulated Tube54
- Simulated Plate54
- Simulated Slide54
- Power Failure During a Run54
Transfer of Protocols from a Block Control Machine55
- Lead Extension55
Optimisation of Protocols55
- Denaturation56
- Annealing56
- Extension56
Chapter 8: Maintenance58
Maintenance58
General Cleaning58
Decontamination58
Protection to the User59
Protection of the Instrument59
- Fuses59
Tube Thermistor Care59
Chapter 9 Technical Specifications & Ordering Information62
Appendix I: Recommended Consumables68
Appendix II: Sealing Systems: the Omniseal Td Mat & Td Tape74
Appendix III: in Situ Block Application Notes74
Appendix IV: Glossary of Terms76

Thermo Hybaid PX2 - Page 2

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