_______________________ Finnigan LTQ Hardware Manual ______________________ 3-1
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Chapter 3
Daily Operation
This chapter outlines the checks and cleaning procedures of the LTQ system
that should be performed every day to insure the proper operation of your
system. This chapter is organized as follows:
• Things to do before operating the LTQ
• Things to do after operating the LTQ
Note. You do not need to tune and calibrate the LTQ as part of your daily
routine.
Calibration parameters are instrument parameters that affect the mass
accuracy and resolution. Tune parameters are instrument parameters that
affect the intensity of the ion signal. You need to tune and calibrate the LTQ
(that is, optimize the tune and calibration parameters) perhaps once a quarter.
Refer to the Automatic Tuning and Calibrating in the ESI/MS Mode
chapter in Finnigan LTQ Getting Started for a procedure for tuning and
calibrating the LTQ. To check the tuning and calibration, follow the
procedure described in the topic Testing the Operation of the MS Detector
in the ESI/MS Mode in Finnigan LTQ Getting Started.
You need to optimize the tune parameters (or change the Tune Method)
whenever you change the type of experiment. Refer to the Optimizing the
MS Detector with Your Compound in ESI/MS Mode or Optimizing the
MS Detector with Your Compound in APCI/MS Mode chapter in
Finnigan LTQ Getting Started for a procedure for optimizing the tune
parameters for your ESI or APCI experiment.
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