Thermo Scientific NanoDrop One User Guide 101
Measure Protein Bradford
Measure Total Protein Concentration
The Protein Bradford assay uses Coomassie Blue dye as a colorimetric detection reagent to
determine total protein concentration in unpurified protein samples. This application is
useful for measuring dilute protein solutions that require lower detection sensitivity or
proteins in the presence of components that exhibit significant absorbance between 200 nm
and 280 nm, which rules out direct protein measurements at 280 nm or 205 nm. This
application measures absorbance at 595 nm and uses a standard curve to calculate protein
concentration. See Working with Standard Curves for more information. A single-point
baseline correction is applied.
Theory of Protein Bradford assay
The Protein Bradford assay uses the protein-induced absorbance shift of Coomassie Blue dye
to determine total protein concentration. The bound protein-dye complex is measured at
595 nm and baseline-corrected using the absorbance value at 750 nm. Pre-formulated kits of
stabilized reagent mixture containing Coomassie Blue dye, alcohol, and surfactant are
available from us or a local distributor.
Measures total protein
concentration of unpurified protein
samples using a Coomassie Blue dye
colorimetric detection reagent.
Measure Total Protein
Reported Results
Settings
Detection Limits
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