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Thermo NanoDrop One - Page 114

Thermo NanoDrop One
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Measure Protein Bradford
108 NanoDrop One User Guide Thermo Scientific
Protein Bradford reported values
The initial screen that appears after each measurement and the standards screen (see previous
image) show a summary of the reported values. To view all reported values, press and hold the
sample row. Here is an example:
Tap row to
select sample
and update
graph; tap more
rows to overlay
abs/conc lines
for up to five
samples. Press
and hold
sample row for
measurement
details.
Drag tab
down/up to see
more/less
sample data
Pinch and zoom to adjust axes;
double-tap to reset
Standard curve
Menu of options;
tap to open
Protein concentration
for selected sample
Absorbance
at 595 nm
Page control; swipe screen left or
right to view next or previous screen
Curve type
R
2
value (1.0
equals perfect fit)
Red squares indicate
sample data points
White circles indicate
standard data points
Selected sample
Note
A baseline correction is performed at 750 nm (absorbance value at 750 nm is
subtracted from absorbance values at all wavelengths in sample spectrum).
Micro-volume absorbance measurements and measurements taken with nonstandard
cuvettes are normalized to a 10.0 mm pathlength equivalent.

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