Measure Protein Lowry
112 NanoDrop One User Guide Thermo Scientific
Protein assay kits and protocols
Follow the assay kit manufacturer’s recommendations for all standards and samples
(unknowns). Ensure each is subjected to the same timing and temperature throughout the
assay.
To measure Protein Lowry standards and samples
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument
arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new
laboratory wipe. For more information, see Cleaning the Pedestals.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.
To measure Protein Lowry standards and samples
1. From the Home screen, select the Proteins tab and tap Protein Lowry.
2. Specify a curve type and number of replicates for each standard and enter the
concentration of each standard.
Tip: For this assay, we recommend setting Curve Type to “2nd Order Polynomial.”
3. Measure blank:
– pipette 2 μL DI H
2
O onto lower pedestal and lower arm, or insert DI H
2
O
blanking cuvette into cuvette holder
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