Thermo Scientific NanoDrop One User Guide 129
Measure OD600
Measure OD600
Use the OD600 application to monitor the growth rate of bacterial or other microbial cell
cultures by measuring the optical density (absorbance) of the culture in growth media at
600 nm. The Beer-Lambert equation and a user-entered conversion factor are used to
correlate absorbance with concentration. Reported concentration values can be used to
identify the phase of cultured cell populations, e.g., log or exponential and stationary.
The OD600 application reports cell concentration in cells/mL. A single-point absorbance
correction can be used. This application does not require a standard curve.
Theory of OD600 application
The OD600 application measures light transmission and uses that value to calculate
absorbance. In spectroscopy, transmitted light is defined as any light that is not absorbed by,
reflected from and scattered off a sample.
Measures the concentration of
microbial cell cultures in solution by
measuring scattered light at 600 nm.
Measure OD600
Reported Results
Settings
Calculations
Note Due to the amount of scattered light present in this assay, absorbance readings are
typically very low.
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