Thermo NanoDrop One - Applications; Detection Limits for All Applications; Chapter 2 Applications

Thermo Scientific NanoDrop One User Guide 9
2
Applications
Detection Limits for All Applications
Detection limits for standard applications
Note Detection limits provided in the tables below are approximate and apply to
micro-volume measurements only; they are based on the instrument’s photometric
absorbance range (10 mm equivalent) of 0–550 A. For measurements with 10 mm
pathlength cuvettes, the photometric absorbance range is 0–1.5 A.
Sample Type Lower Detection Limit Upper Detection Limit Typical Reproducibility
a
dsDNA 2.0 ng/μL (pedestal)
0.20 ng/μL (cuvette)
27,500 ng/μL (pedestal)
75 ng/μL (cuvette)
±2.0 ng/μL for sample concentrations
between 2.0 and 100 ng/μL samples;
±2% for samples >100 ng/μL
ssDNA 1.3 ng/μL (pedestal)
0.13 ng/μL (cuvette)
18,150 ng/μL (pedestal)
49.5 ng/μL (cuvette)
±2.0 ng/μL for sample concentrations
between 2.0 and 100 ng/μL samples;
±2% for samples >100 ng/μL
RNA 1.6 ng/μL (pedestal)
0.16 ng/μL (cuvette)
22,000 ng/μL (pedestal)
60 ng/μL (cuvette)
±2.0 ng/μL for sample concentrations
between 2.0 and 100 ng/μL samples;
±2% for samples >100 ng/μL